KASUMI-2 cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

KASUMI-2 cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

Cell line:
KASUMI-2
Cat no.:
NTCC-DACC526
Species:
human (Homo sapiens)
Cell type:
B cell precursor leukemia
Origin:
established from the bone marrow of a 15-year-old boy with acute lymphoblastic leukemia (ALL FAB L2) in 1990 at diagnosis
Reference(s):
not published
Biosafety level:
1

Cell Culture Data:
Morphology:
small round cells growing singly or in small clumps in suspension, some loosely adherent;
Image:

Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out at ca. 1.5 x 106 cells/ml; start culture in 24-well-plates in the presence of 20% FBS (for the first 1-2 weeks); split saturated culture 1:2 to 1:6 every 2-3 days; maintain at ca. 0.5-2.0 x 106 cells/ml; maximal density of ca. 7 x 106 cells/ml
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 30 hours
Harvest:
cell harvest of ca. 7 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO

Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
CD3 -, CD10 +, CD13 +, CD19 +, CD20 +, CD34 -, CD37 -, CD38 +, CD80 -, HLA-DR +, sm/cyIgG -, smIgM -, cyIgM +, sm/cykappa -, sm/cylamda -;
image：

Fingerprint:
multiplex PCR of minisatellite markers revealed a unique DNA profile
Species:
confirmed as human by cytogenetics
Cytogenetics:
human hypodiploid karyotype with 3% polyploidy - 45(44-46)<2n>X, -Y, del(2)(p23.2/p21p23.1), t(3;14)(q27-28;q24), del(6)(q21), i(7q), del(13)(q1?3q1?4), der(19)t(1;19)(q23;p13) - sideline with idem 46, XY - carries der(19) partner of t(1;19) with TCF3 (E2A) rearrangement confirmed by FISH
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -


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