IEC-18 cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心
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IEC-18 cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心
IEC-18 ( CRL-1589™)Organism: Rattus norvegicus, rat / Cell Type: epithelial / Tissue: ileum / Organism Rattus norvegicus, ratTissue ileumCell Type epithelialProduct Format frozenMorphology epithelialCulture Properties adherentBiosafety Level 1 Derivation IEC-18 was derived from epithelial cells from rat small intestine.Complete Growth Medium Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 0.1 Unit/ml bovine insulin, 95%; fetal bovine serum, 5%Subculturing Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C.Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommendedMedium Renewal: Twice per weekCryopreservation Freeze medium: culture medium 82.5%; fetal bovine serum 10%; DMSO 7.5%Storage temperature: liquid nitrogen vapor phaseCulture Conditions Atmosphere: air, 95%; carbon dioxide (CO2), 5%Temperature: 37°CReferences Quaroni A, et al. Epithelioid cell cultures from rat small intestine. Characterization by morphologic and immunologic criteria. J. Cell Biol. 80: 248-265, 1979. PubMed: 88453
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
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IEC-18 ( CRL-1589™)Organism: Rattus norvegicus, rat / Cell Type: epithelial / Tissue: ileum / Organism Rattus norvegicus, ratTissue ileumCell Type epithelialProduct Format frozenMorphology epithelialCulture Properties adherentBiosafety Level 1 Derivation IEC-18 was derived from epithelial cells from rat small intestine.Complete Growth Medium Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 0.1 Unit/ml bovine insulin, 95%; fetal bovine serum, 5%Subculturing Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C.Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommendedMedium Renewal: Twice per weekCryopreservation Freeze medium: culture medium 82.5%; fetal bovine serum 10%; DMSO 7.5%Storage temperature: liquid nitrogen vapor phaseCulture Conditions Atmosphere: air, 95%; carbon dioxide (CO2), 5%Temperature: 37°CReferences Quaroni A, et al. Epithelioid cell cultures from rat small intestine. Characterization by morphologic and immunologic criteria. J. Cell Biol. 80: 248-265, 1979. PubMed: 88453
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
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