SKW-3 cell line人白血病细胞T细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

SKW-3 cell line人白血病细胞T细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心


Cell line:
SKW-3
DSMZ no.:
ACC 53
Species:
human (Homo sapiens)
Cell type:
T cell leukemia (derivative of KE-37)
Origin:
originally described in the literature to be established from the peripheral blood of a 61-year-old man with T cell chronic lymphocytic leukemia (CLL) in 1977; however, DNA fingerprinting and cytogenetic analysis showed cross-contamination with cell line KE-37; KE-37 was established from a 27-year-old man with acute lymphoblastic leukemia (ALL) in 1979
Reference(s):
14610, 14473
Biosafety level:
1
Permissions and restrictions:
A

DSMZ Cell Culture Data:
Morphology:
single, round cells in suspension; image; image; image
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out at ca. 0.5 x 106 cells/ml; maintain at 0.3-1.0 x 106 cells/ml
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 30-40 hours
Harvest:
saturation density at about 1.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO

DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, PCR assays
Immunology:
CD2 +, cyCD3 +, CD3 -, CD4 +, CD5 +, CD6 +, CD7 +, CD8 +, CD13 -, CD19 -, CD34 -, TCRalpha/beta -, TCRgamma/delta -; image
Fingerprint:
same DNA profile as KE-37 by fluorescent nonaplex PCR of short tandem repeat markers
Species:
confirmed as human with IEF of MDH, NP
Cytogenetics:
human near diploid karyotype with 4% polyploidy - 46(43-48)<2n>XY, +8, -14, t(3;3)(q11;q27), der(8)t(8;14)(q24;q11)x2, t(8;11)(p21;p12), der(12)t(12;?)(q24;?), der(14)t(8;14)(q24;q11)
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -


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