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LIM1215 cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

  • 价  格:¥39865
  • 货  号:LIM1215 cell line细胞株
  • 产  地:北京
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LIM1215 cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

LIM1215 cell line
Cat No.: NTCC-CBA061

Cell Line Designation LIM1215
Expected Cell Viability 96%
Brief Description
Human colorectal carcinoma. Colorectal cancer from HPNCC patient, mutated beta-catenin, secrete TGF-alpha and are responsive to TGFalpha and EGF, A33 positive, MSI
Organism Human (Homo Sapiens)
Tissue Omental metastasis, CRC transcending colon
Growth Properties Adherent
Morphology Epithelial
Image

Growth Medium: RPMI1640 (with 2mM L-Glutamine+25mM HEPES) + 10% FCS, Insulin 0.6μg/ml, Hydrocortisone 1μg/ml, 1-Thioglycerol 10 μM
Subcultivation Ratio Optimal split ratio 1:2-1:4 (seeding density 2 x104 cells/cm2). Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 5 min.

Biosafety Level
PC-2 This cell line is sent with the condition that you are responsible for its safe storage, handling and use.

Additional Information
~1% transfectable with FuGENE, migrate in response to butyrate and grows as a xenograft
References
J Natl Cancer Inst. 1985 Apr;74(4):759-65

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