LIM1215 cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心
- 价 格:¥39865
- 货 号:LIM1215 cell line细胞株
- 产 地:北京
- BioVector NTCC典型培养物保藏中心
- 联系人:Dr.Xu, Biovector NTCC Inc.
电话:400-800-2947 工作微信:1843439339 (QQ同号)
邮件:Biovector@163.com
手机:18901268599
地址:北京
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LIM1215 cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心
LIM1215 cell lineCat No.: NTCC-CBA061Cell Line Designation LIM1215 Expected Cell Viability 96% Brief Description Human colorectal carcinoma. Colorectal cancer from HPNCC patient, mutated beta-catenin, secrete TGF-alpha and are responsive to TGFalpha and EGF, A33 positive, MSI Organism Human (Homo Sapiens) Tissue Omental metastasis, CRC transcending colon Growth Properties Adherent Morphology Epithelial Image Growth Medium: RPMI1640 (with 2mM L-Glutamine+25mM HEPES) + 10% FCS, Insulin 0.6μg/ml, Hydrocortisone 1μg/ml, 1-Thioglycerol 10 μM Subcultivation Ratio Optimal split ratio 1:2-1:4 (seeding density 2 x104 cells/cm2). Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 5 min. Biosafety Level PC-2 This cell line is sent with the condition that you are responsible for its safe storage, handling and use. Additional Information ~1% transfectable with FuGENE, migrate in response to butyrate and grows as a xenograft References J Natl Cancer Inst. 1985 Apr;74(4):759-65
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
LIM1215 cell lineCat No.: NTCC-CBA061Cell Line Designation LIM1215 Expected Cell Viability 96% Brief Description Human colorectal carcinoma. Colorectal cancer from HPNCC patient, mutated beta-catenin, secrete TGF-alpha and are responsive to TGFalpha and EGF, A33 positive, MSI Organism Human (Homo Sapiens) Tissue Omental metastasis, CRC transcending colon Growth Properties Adherent Morphology Epithelial Image Growth Medium: RPMI1640 (with 2mM L-Glutamine+25mM HEPES) + 10% FCS, Insulin 0.6μg/ml, Hydrocortisone 1μg/ml, 1-Thioglycerol 10 μM Subcultivation Ratio Optimal split ratio 1:2-1:4 (seeding density 2 x104 cells/cm2). Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 5 min. Biosafety Level PC-2 This cell line is sent with the condition that you are responsible for its safe storage, handling and use. Additional Information ~1% transfectable with FuGENE, migrate in response to butyrate and grows as a xenograft References J Natl Cancer Inst. 1985 Apr;74(4):759-65
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
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