pCon4-Pro+VH/pCon+VL plasmid抗体表达载体GS谷氨酰胺转移酶加压筛选表达系统质粒 BioVector NTCC质粒载体菌种细胞基因保藏中心
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- 货 号：pCon4-Pro+VH/pCon+VL plasmid抗体表达载体GS谷氨酰胺转移酶加压筛选表达系统质粒
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pCon4-Pro+VH/pCon+VL plasmid抗体表达载体GS谷氨酰胺转移酶加压筛选表达系统质粒 BioVector NTCC质粒载体菌种细胞基因保藏中心
Glutamine Synthetase (GS) gene expression system facilitates the high-level gene expression of proteins using the GS gene as a selectable marker. GS is the enzyme responsible for the biosynthesis of glutamine using glutamate and ammonia as substrates. The activity of the GS enzyme is selectively inhibited by methionine sulphoximine (MSX). Some mammalian cell lines, such as the mouse myeloma NS0 cell line, do not express GS and therefore cannot survive without added glutamine. For these cell lines, a transfected GS gene can function as a selectable marker by permitting growth in a glutamine-free medium. Other cell lines, such as CHO-KI, produce endogenous GS. For these cell lines, glutamine-free medium, containing MSX (at levels sufficient to inhibit the endogenous enzyme) provides the selection pressure. Use of the GS gene expression system for the expression of a recombinant antibody was first described by Bebbington et al., (1992). The aim of the present study was to improve and simplify cloning of variable regions into the GS system, as well as whole antibodies, and thus optimise the rapid generation of high-yielding, stably transfected cell lines expressing monoclonal antibodies.
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
Glutamine Synthetase (GS) gene expression system facilitates the high-level gene expression of proteins using the GS gene as a selectable marker. GS is the enzyme responsible for the biosynthesis of glutamine using glutamate and ammonia as substrates. The activity of the GS enzyme is selectively inhibited by methionine sulphoximine (MSX). Some mammalian cell lines, such as the mouse myeloma NS0 cell line, do not express GS and therefore cannot survive without added glutamine. For these cell lines, a transfected GS gene can function as a selectable marker by permitting growth in a glutamine-free medium. Other cell lines, such as CHO-KI, produce endogenous GS. For these cell lines, glutamine-free medium, containing MSX (at levels sufficient to inhibit the endogenous enzyme) provides the selection pressure. Use of the GS gene expression system for the expression of a recombinant antibody was first described by Bebbington et al., (1992). The aim of the present study was to improve and simplify cloning of variable regions into the GS system, as well as whole antibodies, and thus optimise the rapid generation of high-yielding, stably transfected cell lines expressing monoclonal antibodies.
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
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