McCoy cell line小鼠成纤维细胞系 BioVector NTCC质粒载体菌种细胞基因保藏中心
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- 货 号：McCoy cell line小鼠成纤维细胞系
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McCoy cell line小鼠成纤维细胞系 BioVector NTCC质粒载体菌种细胞基因保藏中心
McCoy [McCoy B] (ATCC® CRL-1696™)Organism: Mus musculus, mouse / Cell Type: fibroblast / Tissue: unknown / Organism Mus musculus, mouseTissue unknownCell Type fibroblastProduct Format frozenMorphology fibroblastCulture Properties adherentBiosafety Level 2Applications The cells have been used to propagate laboratory strains of the 15 recognized serotypes of Chlamydia trachomatis.The cells are susceptible to chlamydia strains, and can be used to propagate chlamydia.Derivation The cells were reported to have originated from the synovial fluid in the knee joint of a patient suffering from degenerative arthritis.A culture of the so-called McCoy cell line was received from the Centers for Disease Control, Cell Culture Department, Atlanta, GA in March, 1984. Documentation as to origin or passage history was not available.Clinical Data The cells were reported to have originated from the synovial fluid in the knee joint of a patient suffering from degenerative arthritis.Comments Little descriptive information about the origin of the McCoy cells appears in the literature. They were first mentioned by Pomerat, et al. Ref. The cells were reported to have originated from the synovial fluid in the knee joint of a patient suffering from degenerative arthritis. In ca. 1965, Defendi, et al., showed that McCoy cells (designated McCoy A) were indeed human cells. However, another subline (designated McCoy B) was, in fact, of mouse origin and possessed marker chromosomes characteristic of strain L mouse fibroblasts. McCoy cells presumed to be human, but which actually are mouse cells, have been disseminated from laboratory to laboratory throughout the world. Initial interest in McCoy cells followed the demonstration by Gordon and Quan Ref and Gordon, et al. Ref that ionizing radiation (cobalt-60) greatly increased the susceptibility of McCoy cells to infection by chlamydia strains. A culture of the so-called McCoy cell line was received from the Centers for Disease Control, Cell Culture Department, Atlanta, GA in March, 1984. Documentation as to origin or passage history was not available. The cells have been used to propagate laboratory strains of the 15 recognized serotypes of Chlamydia trachomatis. The cell line has been satisfactory for chlamydia growth for at least 43 passages at ATCC.The cells are susceptible to chlamydia strains, and can be used to propagate chlamydia.Tested and found negative for ectromelia virus (mousepox).Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.Subculturing Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.Remove and discard culture medium.Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.Add appropriate aliquots of the cell supension to new culture vessels.Incubate cultures at 37°C.Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommendedMedium Renewal: 2 to 3 times per weekCulture Conditions Atmosphere: air, 95%; carbon dioxide (CO2), 5%Temperature: 37°CReferences Pomerat CM, et al. Irradiation of cells in tissue culture. I. Giant cell induction in strain cultures versus elements from primary explants. Z. Zellforsch. 47: 158-174, 1957.
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
McCoy [McCoy B] (ATCC® CRL-1696™)Organism: Mus musculus, mouse / Cell Type: fibroblast / Tissue: unknown / Organism Mus musculus, mouseTissue unknownCell Type fibroblastProduct Format frozenMorphology fibroblastCulture Properties adherentBiosafety Level 2Applications The cells have been used to propagate laboratory strains of the 15 recognized serotypes of Chlamydia trachomatis.The cells are susceptible to chlamydia strains, and can be used to propagate chlamydia.Derivation The cells were reported to have originated from the synovial fluid in the knee joint of a patient suffering from degenerative arthritis.A culture of the so-called McCoy cell line was received from the Centers for Disease Control, Cell Culture Department, Atlanta, GA in March, 1984. Documentation as to origin or passage history was not available.Clinical Data The cells were reported to have originated from the synovial fluid in the knee joint of a patient suffering from degenerative arthritis.Comments Little descriptive information about the origin of the McCoy cells appears in the literature. They were first mentioned by Pomerat, et al. Ref. The cells were reported to have originated from the synovial fluid in the knee joint of a patient suffering from degenerative arthritis. In ca. 1965, Defendi, et al., showed that McCoy cells (designated McCoy A) were indeed human cells. However, another subline (designated McCoy B) was, in fact, of mouse origin and possessed marker chromosomes characteristic of strain L mouse fibroblasts. McCoy cells presumed to be human, but which actually are mouse cells, have been disseminated from laboratory to laboratory throughout the world. Initial interest in McCoy cells followed the demonstration by Gordon and Quan Ref and Gordon, et al. Ref that ionizing radiation (cobalt-60) greatly increased the susceptibility of McCoy cells to infection by chlamydia strains. A culture of the so-called McCoy cell line was received from the Centers for Disease Control, Cell Culture Department, Atlanta, GA in March, 1984. Documentation as to origin or passage history was not available. The cells have been used to propagate laboratory strains of the 15 recognized serotypes of Chlamydia trachomatis. The cell line has been satisfactory for chlamydia growth for at least 43 passages at ATCC.The cells are susceptible to chlamydia strains, and can be used to propagate chlamydia.Tested and found negative for ectromelia virus (mousepox).Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.Subculturing Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.Remove and discard culture medium.Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.Add appropriate aliquots of the cell supension to new culture vessels.Incubate cultures at 37°C.Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommendedMedium Renewal: 2 to 3 times per weekCulture Conditions Atmosphere: air, 95%; carbon dioxide (CO2), 5%Temperature: 37°CReferences Pomerat CM, et al. Irradiation of cells in tissue culture. I. Giant cell induction in strain cultures versus elements from primary explants. Z. Zellforsch. 47: 158-174, 1957.
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
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