pAdenoVator-CMV5-IRES-GFP腺病毒荧光表达载体Ad5型质粒 BioVector NTCC质粒载体菌种细胞基因保藏中心
- 价 格：¥59865
- 货 号：pAdenoVator-CMV5-IRES-GFP腺病毒荧光表达载体Ad5型质粒
- 产 地：北京
- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作微信:1843439339 (QQ同号)
邮件：Biovector@163.com
手机：18901268599
地址：北京
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pAdenoVator-CMV5-IRES-GFP腺病毒荧光表达载体Ad5型质粒 BioVector NTCC质粒载体菌种细胞基因保藏中心
pAdenoVator-CMV5-IRES-GFP is a new Ad transfer vector that combines the sgGFP (SupergloTM Green Fluorescent Protein) with the AdenoVatorTM technology. In this vector the GFP is co-expressed with the gene of interest in an IRES (Internal Ribosome Entry Segment) expression cassette (Mosser et al., 1997). The IRES is a sequence that supports translation initiation from the second cistron in a dicistronic message. In this transfer vector both the gene of interest and the GFP are placed under the control of the modified CMV5 promoter, for very high expression. This arrangement allows for easy visualization of the transduced cells and maximal expression levels with lower MOIs (multiplicity of infection). In addition, inclusion of the GFP in this new transfer vector offers a way to accelerate the identification of Ad recombinants and to facilitate the functional characterization of genes both in vitro and in vivo by allowing easy quantification of gene transfer and expression in a non-invasive manner. Please note that expression levels of the two genes is not equimolar; from our own experience we estimate the proportion of genes expressed to be 3:1 (1 being the gene following the IRES i.e. the GFP in our construct). For Features Location Ad5 LITR: 4-106 Ad5 Encapsidation signal: 197-345 CMV5 promoter/enhancer: 576-1624 IRES: 1654-2372 GFP: 2382-3101 Beta globin Poly A: 3121-3215 Ad5 right homologous sequence: 3251-5249 Ad5 left homologous sequence: 5517-6418 Ad5 RITR: 6521- 6419 pBR322 origin of replication: 6599-7376 Kanamycin resistance: 8201-8995
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
pAdenoVator-CMV5-IRES-GFP is a new Ad transfer vector that combines the sgGFP (SupergloTM Green Fluorescent Protein) with the AdenoVatorTM technology. In this vector the GFP is co-expressed with the gene of interest in an IRES (Internal Ribosome Entry Segment) expression cassette (Mosser et al., 1997). The IRES is a sequence that supports translation initiation from the second cistron in a dicistronic message. In this transfer vector both the gene of interest and the GFP are placed under the control of the modified CMV5 promoter, for very high expression. This arrangement allows for easy visualization of the transduced cells and maximal expression levels with lower MOIs (multiplicity of infection). In addition, inclusion of the GFP in this new transfer vector offers a way to accelerate the identification of Ad recombinants and to facilitate the functional characterization of genes both in vitro and in vivo by allowing easy quantification of gene transfer and expression in a non-invasive manner. Please note that expression levels of the two genes is not equimolar; from our own experience we estimate the proportion of genes expressed to be 3:1 (1 being the gene following the IRES i.e. the GFP in our construct). For Features Location Ad5 LITR: 4-106 Ad5 Encapsidation signal: 197-345 CMV5 promoter/enhancer: 576-1624 IRES: 1654-2372 GFP: 2382-3101 Beta globin Poly A: 3121-3215 Ad5 right homologous sequence: 3251-5249 Ad5 left homologous sequence: 5517-6418 Ad5 RITR: 6521- 6419 pBR322 origin of replication: 6599-7376 Kanamycin resistance: 8201-8995
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
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