WI-38 cell line人胚肺成纤维细胞株WI38 BioVector NTCC质粒载体菌种细胞基因保藏中心

WI-38 cell line人胚肺成纤维细胞株WI38 BioVector NTCC质粒载体菌种细胞基因保藏中心

Organism Homo sapiens, human
Tissue lung
Cell Type fibroblast
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Disease normal
Age 3 months gestation fetus
Gender female
Ethnicity Caucasian
Applications This cell line is a suitable transfection host.
This cell line can be used for viruscide testing.
Karyotype normal diploid
Images
Derivation The WI-38 human diploid cell line was derived by Leonard Hayflick from normal embryonic (3 months gestation) lung tissue.
Clinical Data 3 months gestation fetus
Caucasian
female
Virus Susceptibility Vesicular stomatitis, Glasgow (Indiana)
Herpes simplex virus
Pseudorabies virus
Human poliovirus 1
Comments WI-38 cells have a finite lifetime of 50 plus or minus 10 population doublings with a doubling time of 24 hours.
This line was the first human diploid cell line to be used in human vaccine preparation.
The 8th passage ampule from which this freeze was derived was found to contain a bacterial contaminant (a micrococcus). The cell line was subsequently cured by several passages in the presence of antibiotics.
Growth of the cells is enhanced by addition of tumor necrosis factor alpha (TNF alpha) to the medium.
This cell line is negative for reverse transcriptase.
Complete Growth Medium The base medium for this cell line is Eagle's Minimum Essential Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Corning® T-75 flasks are recommended for subculturing this product.
1.Remove and discard culture medium.
2.Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
3.Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).  Note: To avoid clumping do not agitate the cells by hitting or   shaking the flask while waiting for the cells to detach.  Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. 
4.Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. 
5.Add appropriate aliquots of cell suspension to new culture vessels
6.Place culture vessels in incubators at 37°C.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation growth medium, 95%; DMSO, 5%
Culture Conditions Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
STR Profile Amelogenin: X
CSF1PO: 10,12
D13S317: 11
D16S539: 11,12
D5S818: 10
D7S820: 9,11
TH01: 8,9.3
TPOX: 8
vWA: 19,20
Isoenzymes G6PD, B
Population Doubling Time 24 hrs
Passage History The 8th passage ampule from which this freeze was derived was found to contain a bacterial contaminant (a micrococcus). The cell line was subsequently cured by several passages in the presence of antibiotics.
References Sugarman BJ, et al. Recombinant human tumor necrosis factor-alpha: effects on proliferation of normal and transformed cells in vitro. Science 230: 943-945, 1985. PubMed: 3933111

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