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HSC-F cell line食蟹猴胚胎脾脏淋巴T细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

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  • 货  号:HSC-F cell line食蟹猴胚胎脾脏淋巴T细胞株
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HSC-F cell line食蟹猴胚胎脾脏淋巴T细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

Cell Name HSC-F
Profile T-cell line (fetal spleen-derived lymphocytes) of cynomolgus monkey. Other Name
Animal monkey, Cynomolgus Strain Cynomolgus
Genus Macaca Species fascicularis
Sex * Age fetal
Identity not done Tissue for Primary Cancer hemo-lymphocytic
Case history Metastasis
Tissue Metastasized Genetics Positive for CD2, CD3, CD4, CD8a, CD25, CD28, CD29, CD38, CD40L, CD44, CD49d, CD58, CD69, CD70, CD80, CD95 & MHC-IIDR.
Life Span infinite Crisis PDL
Morphology lymphocyte-like Character Scattered clumps observed in cell culture and this cell line is liable to apotosis due to overgrowth. Herpesvirus saimiri is latently infected, while no infectious virus is produced from this cell line.
Classify transformed Established by Akari,H.
Registered by Akari,H. Regulation for Distribution
Comment This cell line dose not require hIL-2, but in case of poor condition of cell growth an addition of the hIL-2(50U/ml)will improve the cell growth. Year 2006
Medium RPMI-1640 with 10% fetal bovine serum. Methods for Passages suspension culture (dilute 1/5) in 10ml of T25 flask vertical culture.
Cell Number on Passage 1x10^6 cells/ml in 10mL of T25 Race
CO2 Conc. 5 % Tissue Sampling spleen-derived lymphocytes
Virus Summary Virus Tested
Tissue Type
Link
Cellosaurus(ExPASy) CVCL_5485
Reference
Pubmed id:10370378 Effects of SIVmac infection on peripheral blood CD4+CD8+ T lymphocytes in cynomolgus macaques.
Akari H,Nam KH,Mori K,Otani I,Shibata H,Adachi A,Terao K,Yoshikawa Y
Clin Immunol. 1999 Jun;91(3):321-9
Pubmed id:8610465 In vitro immortalization of Old World monkey T lymphocytes with Herpesvirus saimiri: its susceptibility to infection with simian immunodeficiency viruses.
Akari H,Mori K,Terao K,Otani I,Fukasawa M,Mukai R,Yoshikawa Y
Virology. 1996 Apr 15;218(2):382-8

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