GLP1R/CRE/LUCI HEK293 cell line稳转细胞株GPCR靶点 BioVector NTCC质粒载体菌种细胞基因保藏中心
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- 货 号：GLP1R/CRE/LUCI HEK293 cell line稳转细胞株GPCR靶点
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GLP1R/CRE/LUCI HEK293 cell line稳转细胞株GPCR靶点 BioVector NTCC质粒载体菌种细胞基因保藏中心
BackgroundGLP1R binds specifically the glucagon-like peptide-1 (GLP1) and has much lower affinity for related peptides such as the gastric inhibitory polypeptide and glucagon.GLP1R is known to be expressed in pancreatic beta cells. Activated GLP1R stimulates the adenylyl cyclase pathway which results in increased insulin synthesis and release of insulin. Consequently, GLP1R has been suggested as a potential target for the treatment of diabetes.GLP1R is also expressed in the brain where it is involved in the control of appetite. Furthermore, mice which over express GLP1R display improved memory and learning. II. IntroductionHost Cell:HEK293Expressed gene:Genbank Accession Number NM_002062; no expressed tagsStability:16 passagesSynonym(s):GLP-1Freeze Medium:45% culture medium, 45% FBS, 10% DMSOCulture Medium:DMEM, 10% FBS, 500 μg/ml G418Mycoplasma Status:NegativeStorage:Liquid nitrogen immediately upon deliveryApplication(s):Functional assay for GLP1 receptor Ⅲ. Description of Host Cell LineOrganism:HumanTissue:Embryonic kidneyDisease:NormalMorphology:Epitheloid cellGrowth Properties:Adherent Ⅳ.Representative DataFigure 1. GLP-1 (7-37)-induced concentration-dependent stimulation of intracellular luciferase in HEK293/GLP1R/Luc and HEK293 cells. After stimulation with GLP1 receptor agonist, GLP-1 (7-37), the cells are checked with BrightGloLuciferase Assay System and the relative luminescence units (RLU) of luciferase were recorded on a FlexStation. The RLU were plotted against the log of the cumulative doses (10 -fold dilution) of GLP-1 (7-37) (Mean ± SD, n = 2). The EC50 of GLP-1 (7-37) co-expressing with Luc in HEK293 cells was 4.91 nM.
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
BackgroundGLP1R binds specifically the glucagon-like peptide-1 (GLP1) and has much lower affinity for related peptides such as the gastric inhibitory polypeptide and glucagon.GLP1R is known to be expressed in pancreatic beta cells. Activated GLP1R stimulates the adenylyl cyclase pathway which results in increased insulin synthesis and release of insulin. Consequently, GLP1R has been suggested as a potential target for the treatment of diabetes.GLP1R is also expressed in the brain where it is involved in the control of appetite. Furthermore, mice which over express GLP1R display improved memory and learning. II. IntroductionHost Cell:HEK293Expressed gene:Genbank Accession Number NM_002062; no expressed tagsStability:16 passagesSynonym(s):GLP-1Freeze Medium:45% culture medium, 45% FBS, 10% DMSOCulture Medium:DMEM, 10% FBS, 500 μg/ml G418Mycoplasma Status:NegativeStorage:Liquid nitrogen immediately upon deliveryApplication(s):Functional assay for GLP1 receptor Ⅲ. Description of Host Cell LineOrganism:HumanTissue:Embryonic kidneyDisease:NormalMorphology:Epitheloid cellGrowth Properties:Adherent Ⅳ.Representative DataFigure 1. GLP-1 (7-37)-induced concentration-dependent stimulation of intracellular luciferase in HEK293/GLP1R/Luc and HEK293 cells. After stimulation with GLP1 receptor agonist, GLP-1 (7-37), the cells are checked with BrightGloLuciferase Assay System and the relative luminescence units (RLU) of luciferase were recorded on a FlexStation. The RLU were plotted against the log of the cumulative doses (10 -fold dilution) of GLP-1 (7-37) (Mean ± SD, n = 2). The EC50 of GLP-1 (7-37) co-expressing with Luc in HEK293 cells was 4.91 nM.
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
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