OCI-LY1 cell line人弥漫大B细胞淋巴瘤细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心
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- 货 号：OCI-LY1 cell line人弥漫大B细胞淋巴瘤细胞株
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OCI-LY1 cell line人弥漫大B细胞淋巴瘤细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心
OCI-LY1DSMZ no.:ACC 722Species:human (Homo sapiens)Cell type:B cell lymphomaOrigin:established from the bone marrow of a 44-year-old man with B-cell non-Hodgkin lymphoma (B-NHL; diffuse large cell), stage 4B at relapse in 1983; assigned to GCB-like lymphoma subtype (germinal center B-cell)Reference(s):14427, 15976, 16454, 16455, 16456, 17354Biosafety level:1Permissions and restrictions:A DSMZ Cell Culture Data:Morphology:small round to polymorph cells growing singly in suspension; image; image; imageMedium:80% Iscove's MDM + 20% h.i. FBSSubculture:seed out at ca. 0.5 x 106 cells/ml; maintain at ca. 0.5-2 x 106 cells/ml; split saturated culture 1:2 every 2-3 daysIncubation:at 37 °C with 5% CO2Doubling time:ca. 50 hoursHarvest:maximum density at ca. 2.5 x 106 cells/mlStorage:frozen with 70% medium, 20% FBS, 10% DMSO DSMZ Scientific Data:Mycoplasma:negative in microbiological culture, PCR assaysImmunology:CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, CD138 -, HLA-DR -, cyIgG -, cyIgM +, cykappa +, cylambda -; imageFingerprint:fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profileCytogenetics:human flat-moded hyperdiploid karyotype with 6% polyploidy; 47(41-48)<2n>XY, +7, der(4)t(4;7)(p14;q22), der(7)t(4;7)(p14;p14)del(7)(q22), der(8)t(8;13)(p13;q13), der(8)t(8;8)(p22;q11), der(10)t(10;14)(p14;q32)t(14;11)(q32;q24), der(11)t(11;14)(q24;q32)t(14;18)(q32;q21), del(13)(q31), t(14;18)(q32;q21), der(18)t(3;18)(q24;q21)t(18;18)(p12;q21); carries t(14;18) rearrangements, some cryptic, effecting juxtaposition of IGH with BCL2; resembles published karyotypeViruses:PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
OCI-LY1DSMZ no.:ACC 722Species:human (Homo sapiens)Cell type:B cell lymphomaOrigin:established from the bone marrow of a 44-year-old man with B-cell non-Hodgkin lymphoma (B-NHL; diffuse large cell), stage 4B at relapse in 1983; assigned to GCB-like lymphoma subtype (germinal center B-cell)Reference(s):14427, 15976, 16454, 16455, 16456, 17354Biosafety level:1Permissions and restrictions:A DSMZ Cell Culture Data:Morphology:small round to polymorph cells growing singly in suspension; image; image; imageMedium:80% Iscove's MDM + 20% h.i. FBSSubculture:seed out at ca. 0.5 x 106 cells/ml; maintain at ca. 0.5-2 x 106 cells/ml; split saturated culture 1:2 every 2-3 daysIncubation:at 37 °C with 5% CO2Doubling time:ca. 50 hoursHarvest:maximum density at ca. 2.5 x 106 cells/mlStorage:frozen with 70% medium, 20% FBS, 10% DMSO DSMZ Scientific Data:Mycoplasma:negative in microbiological culture, PCR assaysImmunology:CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, CD138 -, HLA-DR -, cyIgG -, cyIgM +, cykappa +, cylambda -; imageFingerprint:fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profileCytogenetics:human flat-moded hyperdiploid karyotype with 6% polyploidy; 47(41-48)<2n>XY, +7, der(4)t(4;7)(p14;q22), der(7)t(4;7)(p14;p14)del(7)(q22), der(8)t(8;13)(p13;q13), der(8)t(8;8)(p22;q11), der(10)t(10;14)(p14;q32)t(14;11)(q32;q24), der(11)t(11;14)(q24;q32)t(14;18)(q32;q21), del(13)(q31), t(14;18)(q32;q21), der(18)t(3;18)(q24;q21)t(18;18)(p12;q21); carries t(14;18) rearrangements, some cryptic, effecting juxtaposition of IGH with BCL2; resembles published karyotypeViruses:PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
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