pSinRep5辛德毕斯病毒表达载体质粒 BioVector NTCC质粒载体菌种细胞基因保藏中心

pSinRep5辛德毕斯病毒表达载体质粒 BioVector NTCC质粒载体菌种细胞基因保藏中心

pSinRep5 is a 9951 bp vector used to generate recombinant RNA molecules for transfection or infection of eukaryotic cell lines. It contains the Sindbis virus nonstructural protein genes 1-4 (nsP1-4) for replicating RNA transcripts in vivo, the promoter for subgenomic transcription, and a multiple cloning site. The multiple cloning site (Xba I, Mlu I, Pml I, Sph I, Stu I, and Apa I) allows insertion of your gene of interest behind the subgenomic promoter for expression in BHK cells or the cell line of choice. Three unique restriction sites (Xho I, Not I, and Pac I) located 3´ to the multiple cloning site allow linearization of the plasmid prior to in vitro transcription. The recombinant RNA transcripts are synthesized using the SP6 promoter and transfected into BHK cells.

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