Murine hepatitis virus鼠肺炎病毒SARS相关毒株 BioVector NTCC质粒载体菌种细胞基因保藏中心
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- 货 号：Murine hepatitis virus鼠肺炎病毒SARS相关毒株
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电话：400-800-2947 工作微信:1843439339 (QQ同号)
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Murine hepatitis virus鼠肺炎病毒SARS相关毒株 BioVector NTCC质粒载体菌种细胞基因保藏中心
Classification Nidovirales, Coronaviridae, Coronavirus,Group 2Agent Murine hepatitis virusStrain MHV-A59Common Name Murine hepatitis virusBiosafety Level 2Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.Isolation Pool of liver and spleen suspension of BALB/c mice exhibiting rough coats and hunched positionProduct Format frozenStorage Conditions -70°C or colder (or colder)Comments The MHV-A59 virus was isolated from BALB/c mice in which Moloney murine leukemia had been serially passaged by i.p. injection of homogenates of liver, spleen, thymus and lymph node. It is possible that the preparation still contains murine leukemia virus.Effect on Host Yes, in vitro effects: Cytopathic effects in tissue cultureCPE, rounding and sloughing, attached and floating syncytial debrisGrowth Medium NCTC 135 medium, 90%; horse serum, 10% Recommended Host NCTC clone 1469 cells (ATCC CCL-9.1)Recommended Host: NCTC 1469 (ATCC CCL-9.1)Growth Conditions Incubation: 18 to 24 hours at 37°C, a 5%CO2 in air atmosphere is recommendedRecommendations for Infection: For best results cells should be 24 to 48 hours old and 80% - 90% confluent (not 100% confluent).Temperature: 37°CReferences Piczak CV, et al. A hepatitis virus complicating studies with mouse leukemia. J. Natl. Cancer Inst. 27: 29-51, 1961. PubMed: 13766009rounding and sloughing with attached and floating syncytial debris
【Supplier来源】BioVector NTCC Inc.
TEL：+86-010-53513060
【Website网址】 http://www.biovector.net
Classification Nidovirales, Coronaviridae, Coronavirus,Group 2Agent Murine hepatitis virusStrain MHV-A59Common Name Murine hepatitis virusBiosafety Level 2Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.Isolation Pool of liver and spleen suspension of BALB/c mice exhibiting rough coats and hunched positionProduct Format frozenStorage Conditions -70°C or colder (or colder)Comments The MHV-A59 virus was isolated from BALB/c mice in which Moloney murine leukemia had been serially passaged by i.p. injection of homogenates of liver, spleen, thymus and lymph node. It is possible that the preparation still contains murine leukemia virus.Effect on Host Yes, in vitro effects: Cytopathic effects in tissue cultureCPE, rounding and sloughing, attached and floating syncytial debrisGrowth Medium NCTC 135 medium, 90%; horse serum, 10% Recommended Host NCTC clone 1469 cells (ATCC CCL-9.1)Recommended Host: NCTC 1469 (ATCC CCL-9.1)Growth Conditions Incubation: 18 to 24 hours at 37°C, a 5%CO2 in air atmosphere is recommendedRecommendations for Infection: For best results cells should be 24 to 48 hours old and 80% - 90% confluent (not 100% confluent).Temperature: 37°CReferences Piczak CV, et al. A hepatitis virus complicating studies with mouse leukemia. J. Natl. Cancer Inst. 27: 29-51, 1961. PubMed: 13766009rounding and sloughing with attached and floating syncytial debris
【Supplier来源】BioVector NTCC Inc.
TEL：+86-010-53513060
【Website网址】 http://www.biovector.net
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