pNBU2-bla-ermGb染色体插入质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心
- 价 格：¥49865
- 货 号：pNBU2-bla-ermGb染色体插入质粒载体
- 产 地：北京
- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作微信:1843439339 (QQ同号)
邮件：Biovector@163.com
手机：18901268599
地址：北京
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pNBU2-bla-ermGb染色体插入质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心
pNBU2-bla-ermGb chromosomal insertion vector (Structure 16: 1-11). Derivative of pKNOCK-bla-ermGb into which a PCR-amplifiedintN2-att fragment (J. Bacteriol. 182: 3559-71) was inserted using engineered SalI and KpnI ends. MCS sites can be used to insert fragmentsinto pKNOCK-Nbu2-ermb and the entire plasmid construct can then be conjugated into Bacteroides spp. and insertions made into either of thetwo, NBU2-targeted, tRNAser loci. Uses include complementation of IDM, deletion or transposon mutants and introduction of exogenous DNAfragments into the genomes of Bacteroides spp. Select restriction sites are shown along with the multiple cloning site (MCS), R6K replicationorigin (oriR6K) and the RP4 transfer origin (RP4 oriT).
【Supplier来源】BioVector NTCC Inc.
TEL：+86-010-53513060
【Website网址】 http://www.biovector.net
pNBU2-bla-ermGb chromosomal insertion vector (Structure 16: 1-11). Derivative of pKNOCK-bla-ermGb into which a PCR-amplifiedintN2-att fragment (J. Bacteriol. 182: 3559-71) was inserted using engineered SalI and KpnI ends. MCS sites can be used to insert fragmentsinto pKNOCK-Nbu2-ermb and the entire plasmid construct can then be conjugated into Bacteroides spp. and insertions made into either of thetwo, NBU2-targeted, tRNAser loci. Uses include complementation of IDM, deletion or transposon mutants and introduction of exogenous DNAfragments into the genomes of Bacteroides spp. Select restriction sites are shown along with the multiple cloning site (MCS), R6K replicationorigin (oriR6K) and the RP4 transfer origin (RP4 oriT).
【Supplier来源】BioVector NTCC Inc.
TEL：+86-010-53513060
【Website网址】 http://www.biovector.net
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