MV-4-11-Luc cell line荧光稳转细胞株MV-4-11Luc BioVector NTCC质粒载体菌种细胞基因保藏中心
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- 货 号：MV-4-11-Luc cell line荧光稳转细胞株MV-4-11Luc
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MV-4-11-Luc cell line荧光稳转细胞株MV-4-11Luc BioVector NTCC质粒载体菌种细胞基因保藏中心
MV4–11-luc cells, which exogenously express firefly luciferase, are used in the orthotopic in vivo model.Organism Homo sapiens, humanTissue peripheral bloodCell Type macrophageProduct Format frozenMorphology lymphoblastCulture Properties suspensionBiosafety Level 1Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.Disease biphenotypic B myelomonocytic leukemiaAge 10 yearsGender maleApplications This cell line is a suitable transfection host.Karyotype 48, XY, t(4;11)(q21;q23), +8, +19Derivation The MV4-11 cell line was established by Rovera and associates from the blast cells of a 10-year-old male with biphenotypic B-myelomonocytic leukemia. The growth factor, granulocyte/macrophage colony-stimulating factor (GM-CSF), was required to establish this cell line and growth factors are necessary for its continuous proliferation in chemically defined medium [PubMed: 3496132].Clinical Data maleThe MV4-11 cell line was established by Rovera and associates from the blast cells of a 10-year-old male with biphenotypic B-myelomonocytic leukemia.Antigen Expression CD4; Homo sapiensCD10; Homo sapiensCD15, human; Homo sapiensCD4 (40-96%); CD10 (4-11%); CD15 (96-99%)Genes Expressed CD4; Homo sapiens, CD10; Homo sapiens, CD15, human; Homo sapiens, CD4 (40-96%); CD10 (4-11%); CD15 (96-99%)Comments This line can be propagated in medium supplemented with 10% FBS without the addition of growth factors. IL-3 can independently support the long-term growth of this cell line, but IL-3 antagonized the proliferation of MV4-11 cells in the presence of GM-CSF when both factors were used at very low concentrations. Granulocyte colony stimulating factor (G-CSF) synergized with GM-CSF in inducing proliferation of MV4-11 cells; G-CSF alone causes a transient stimulation of the cell line.This line was formerly designated ATCC HTB-189.Over 96% of these cells are positive by indirect immunofluorescence for the myelomonocytic antigen CD15, 40-96% are positive for the monocytic antigen CD4, and 4-11% are positive for CD10 [PubMed: 3500218].Complete Growth Medium The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.Subculturing Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 105 cells/mL and maintain between 1 X 105 and 1 X 106 cells/mL. Corning® T-75 flasks (catalog #431464) are recommended for subculturing this product.Medium Renewal: Every 2 to 3 daysNote: If the cells are maintained in a serum-free medium, it is necessary to add the following: 0.005 mg/mL transferrin, 0.005 mg/mL insulin, and 5 ng/mL GM-CSF.Cryopreservation Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSOStorage temperature: liquid nitrogen vapor phaseCulture Conditions Atmosphere: air, 95%; carbon dioxide (CO2), 5%Temperature: 37°CSTR Profile Amelogenin: X,YCSF1PO: 10,12D13S317: 13D16S539: 11,12D5S818: 11,12D7S820: 8,9TH01: 8,9.3TPOX: 8,11vWA: 14,15U.S. Patent Number 5,516,512
【Supplier来源】BioVector NTCC Inc.
TEL：+86-010-53513060
【Website网址】 http://www.biovector.net
MV4–11-luc cells, which exogenously express firefly luciferase, are used in the orthotopic in vivo model.Organism Homo sapiens, humanTissue peripheral bloodCell Type macrophageProduct Format frozenMorphology lymphoblastCulture Properties suspensionBiosafety Level 1Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.Disease biphenotypic B myelomonocytic leukemiaAge 10 yearsGender maleApplications This cell line is a suitable transfection host.Karyotype 48, XY, t(4;11)(q21;q23), +8, +19Derivation The MV4-11 cell line was established by Rovera and associates from the blast cells of a 10-year-old male with biphenotypic B-myelomonocytic leukemia. The growth factor, granulocyte/macrophage colony-stimulating factor (GM-CSF), was required to establish this cell line and growth factors are necessary for its continuous proliferation in chemically defined medium [PubMed: 3496132].Clinical Data maleThe MV4-11 cell line was established by Rovera and associates from the blast cells of a 10-year-old male with biphenotypic B-myelomonocytic leukemia.Antigen Expression CD4; Homo sapiensCD10; Homo sapiensCD15, human; Homo sapiensCD4 (40-96%); CD10 (4-11%); CD15 (96-99%)Genes Expressed CD4; Homo sapiens, CD10; Homo sapiens, CD15, human; Homo sapiens, CD4 (40-96%); CD10 (4-11%); CD15 (96-99%)Comments This line can be propagated in medium supplemented with 10% FBS without the addition of growth factors. IL-3 can independently support the long-term growth of this cell line, but IL-3 antagonized the proliferation of MV4-11 cells in the presence of GM-CSF when both factors were used at very low concentrations. Granulocyte colony stimulating factor (G-CSF) synergized with GM-CSF in inducing proliferation of MV4-11 cells; G-CSF alone causes a transient stimulation of the cell line.This line was formerly designated ATCC HTB-189.Over 96% of these cells are positive by indirect immunofluorescence for the myelomonocytic antigen CD15, 40-96% are positive for the monocytic antigen CD4, and 4-11% are positive for CD10 [PubMed: 3500218].Complete Growth Medium The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.Subculturing Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 105 cells/mL and maintain between 1 X 105 and 1 X 106 cells/mL. Corning® T-75 flasks (catalog #431464) are recommended for subculturing this product.Medium Renewal: Every 2 to 3 daysNote: If the cells are maintained in a serum-free medium, it is necessary to add the following: 0.005 mg/mL transferrin, 0.005 mg/mL insulin, and 5 ng/mL GM-CSF.Cryopreservation Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSOStorage temperature: liquid nitrogen vapor phaseCulture Conditions Atmosphere: air, 95%; carbon dioxide (CO2), 5%Temperature: 37°CSTR Profile Amelogenin: X,YCSF1PO: 10,12D13S317: 13D16S539: 11,12D5S818: 11,12D7S820: 8,9TH01: 8,9.3TPOX: 8,11vWA: 14,15U.S. Patent Number 5,516,512
【Supplier来源】BioVector NTCC Inc.
TEL：+86-010-53513060
【Website网址】 http://www.biovector.net
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