8E5 cell line人外周血淋巴母细胞HIV人类免疫缺陷病毒阳性细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

8E5 cell line人外周血淋巴母细胞HIV人类免疫缺陷病毒阳性细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心
8E5 cell line Organism: Homo sapiens, human / Cell Type: lymphoblast human immunodeficiency virus (HIV) positive / Tissue: peripheral blood / Disease: acute lymphoblastic leukemia Cat No.: NTCC-8993 Organism Homo sapiens, human Tissue peripheral blood Cell Type lymphoblast human immunodeficiency virus (HIV) positive Product Format frozen Morphology lymphoblast Culture Properties suspension Biosafety Level 2 [Cells contain Retrovirus] Disease acute lymphoblastic leukemia Age 4 years Gender female Ethnicity Caucasian Clinical Data female Caucasian 4 years Antigen Expression CD4 +; CD5 + Receptor Expression transferrin Genes Expressed most human immunodeficiency virus (HIV, HTLV III, LAV) structural proteins Cellular Products most human immunodeficiency virus (HIV, HTLV III, LAV) structural proteins Comments The cells contain a single defective proviral genome of HTLV III (HIV, LAV). All of the major structural protein are constitutively expressed with the exception of the p64 and p34 proteins. No infectious virus is produced; however, co-cultivation with Leu-3 + cells leads to syncytia formation. The cells have been cured of a mycoplasma contamination. Complete Growth Medium The base medium for this cell line is RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Subculturing Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 105 viable cells/mL. Maintain cultures at a cell concentration between 1 x 105 and 1 x 106 cells/mL. Medium Renewal: Add fresh medium (20% to 30% by volume) every 2 to 3 days. Cryopreservation Complete growth medium 95%; DMSO, 5%. Culture Conditions Temperature: 37°C
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