INS-1 832/13 cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

INS-1 832/13 cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心
INS-1 832/3 rat insulinoma cell line is a useful model for insulin secretion regulation and pancreatic islet beta-cell function studies.
Glucose-stimulated insulin secretion (GSIS) is potentiated by pancreatic beta cells and is critical to the physiological control of blood glucose levels. Insulin secretion is impaired in type 2 diabetes, and insight into the mechanisms and regulation of insulin secretion is fundamental to understanding the roles of beta cells in metabolic disease. References: 1. Endocrinology 1992; 130(1): 167-178. 2. Diabetes 2000; 49(3): 424-30. 3. EMBO J 1997; 16(19):5837-5846. The rat insulinoma cell line INS-1 is a well-established model for studies of pancreatic islet beta-cell function (1); however, the GSIS response of INS-1 cells may decrease over time. The INS-1 832/13 cell line is a subclone of INS-1 that was selected for robust GSIS, producing and secreting both rat and human insulin. INS-1 832/13 harbors a human insulin expression cassette allowing for human insulin secretion to be maintained over extended passages with selection (2). INS-1 832/13 cells exhibit enhanced secretory responsiveness to glucose as compared with the parental INS-1 cell line (2). INS-1 832/13 cells may be characterized by granular staining for synaptotagmin, as described for the parental cell line (3). These characteristics make INS-1 832/13 cells a useful system for investigating mechanisms of cellular insulin secretion, storage and synthesis. Source: INS-1 832/13 is a derivative of INS-1 cells originally established from an x-ray induced insulinoma in rat (1). The INS-1 832/13 cell line is a subclone of INS-1 that was stably transfected with a CMV promoter-human insulin expression plasmid carrying a geneticin (G418)-resistance marker for selection (2). Cell Line Description Cancer Cells Application INS-1 832/3 rat insulinoma cell line is a useful model for insulin secretion regulation and pancreatic islet beta-cell function studies. Research Category Metabolism Research Sub Category Diabetes
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