pNCMO2 plasmid vector短短芽孢杆菌穿梭表达载体质粒 BioVector NTCC质粒载体菌种细胞基因保藏中心

pNCMO2 plasmid vector短短芽孢杆菌穿梭表达载体质粒 BioVector NTCC质粒载体菌种细胞基因保藏中心
pNCMO2 is a Brevibacillus and E. coli shuttle vector. Accordingly, the expression plasmid is constructed in E. coli and then is introduced into Brevibacillus for protein expression. The P2 promoter, derived from a cell-wall protein of the host bacterium, is used as the expression promoter for pNCMO2. Because the P2 promoter is not functional in E. coli, it is useful for cloning the target gene. However, it is an exceptionally strong pro-moter in Brevibacillus and results in efficient protein production. This strong promoter activity may inhibit the growth of transformants in some cases. If this occurs, consider using the pNY326 vector
Vector Information for pNCMO2>P2 PromoterUses part of the 5' sequence of the cell-wall protein gene. Has almost no activity in E. coli.Very strong expression in Brevibacillus. Sec SignalA modified secretion signal to increase versatilityMultiple Cloning Site11 restriction enzyme cutting sitesX-TerminatorA 46-bp terminator structure downstream from the multiple cloning siteRepGene relating to plasmid replication (from pUB110)OriReplication origin for replication of the plasmid in Brevibacillus(from pUB110)NmrNeomycin resistance geneSelection marker in BrevibacillusColE1 OriOrigin of replication for the plasmid in E. coli (pUC derived)AmprAmpicillin resistance geneSelection marker in E. coli
pNCMO2是Brevibacillus 和E. coli 间的穿梭质粒。在E. coli 中构建表达质粒后，转入Brevibacillus 进行蛋白质表达。 宿主菌细胞壁蛋白质的P2启动子作为pNCMO2的表达启动子，由于P2启动子在E. coli 中不起作用，有利于克隆目的基因；但在Brevibacillus 中则是呈指数级别的强启动子，可有效生产蛋白质。
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