pGacIJ等原核表达载体质粒 BioVector NTCC质粒载体菌种细胞基因保藏中心

pGacIJ等原核表达载体质粒 BioVector NTCC质粒载体菌种细胞基因保藏中心
MGAS5005 GAS, M1-serotype strain (1) 5005ΔgacI MGAS5005 has a 82-nt deletion in gacI. This study 5005ΔgacL MGAS5005 has a nonpolar aadA spectinomycin resistance cassette inserted in gacL This study 5005ΔgacL gacL+ 5005ΔgacL has pGacL integrated into the chromosome This study GBS COH1 A clinical isolate of GBS from a case of fatal infant septicemia belonging to the hypervirulent ST-17 lineage (2) COH1ΔgacI GBS COH1 has a nonpolar aadA spectinomycin resistance cassette inserted in gacI This study Bacillus cereus ATCC 14579 ATCC E. coli DH5α E. coli cells used for cloning Invitrogen E. coli Rosetta (DE3) E. coli cells used for protein expression Novagen CLM37 E. coli W3310 derivative has deletion in wecA (3) CLM37:GacO E. coli CLM37 carrying pGacO This study PR4019 E. coli K-12 expressing WecA (4) Plasmids pLR16T Streptococcus-E. coli shuttle vector (5) pBAD33 E. coli protein expression vector harboring arabinoseinducible promoter ATCC pJRS233 E. coli - Streptococcus temperature-sensitive shuttle vector for allelic replacement (6) pJRS233ΔgacI Derivative of pJRS233 containing a 1.4 kbp DNA fragment carrying GAS gacI locus. GacI has a 82-nt deletion This study pUC19BX Derivative of pUC19 (NE BioLabs) containing BglII and XhoI cloning sites in the polylinker region This study pUC19BXspec Derivative of pUC19BX expressing aadA with own rbs This study pUC19BXspecL1 Derivative of pUC19BXspec containing gacL 5’ region This study pUC19BXspecL2 Derivative of pUC19BXspec expressing aadA flanked with gacL 5’ and 3’ regions This study pHY304 A temperature sensitive E.coli-Streptococcus shuttle vector (7) pHY304ΔgacL Derivative of pHY304 expressing aadA flanked with gacL 5’ and 3’ regions This study pHY304GBSΔI Derivative of pHY304 expressing aadA flanked with GBS gacI 5’ and 3’ regions This study pBBL740 S. pyogenes integrational plasmid (8) pGacL pBBL740 derived plasmid expressing gacL This study pRSF-NT A modified pRSF-Duet1 (Novagen) vector that allows the creation of N-terminus His-tagged proteins with a TEV protease cleavage site (9) pGacI pRSF-NT derived plasmid expressing GAS gacI fused with N-terminal TEV protease cleavable His-tag This study pET21_NESG pET21 derived plasmid expressing epaI with His-tag at its Cterminus DNASU (10) pGacIJ Derivative of pRSF-NT expressing GAS gacI fused with Nterminal His-tag followed by gacJ This study pGacO pBAD33 derived plasmid expressing gacO This study pCbpD pRSF-NT derived plasmid expressing cbpD fused with Nterminal TEV protease cleavable His-tag This study pPlyPy pET-21d (Novagen) derived plasmid expressing M5005_Spy_1001 gene fused with C-terminal His-tag This study plyCB pET-21d (Novagen) derived plasmid expressing plyCA followed by plyCB fused at the C terminus with a His-tag This study
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