Spa’-pNK3260短芽孢杆菌表达载体Bacillus brevis质粒 BioVector NTCC质粒载体菌种细胞基因保藏中心

Spa’-pNK3260短芽孢杆菌表达载体Bacillus brevis质粒 BioVector NTCC质粒载体菌种细胞基因保藏中心
The present invention provides a method for producing a recombinant protein, which can increase the cell growth rate in culture while stably maintaining plasmids in the cells, thereby improving the recombinant protein yield. The present invention provides a method for producing a recombinant protein, including a high-temperature culture step of culturing at 32° C. or higher a Brevibacillus bacterium that contains a gene encoding a recombinant protein, and a low-temperature culture step of culturing the Brevibacillus bacterium at a temperature lower than 32° C. after the high-temperature culture step.
FIG. 1 is a schematic view of expression plasmid Spa′-pNK3260. FIG. 2 shows the base sequences of a promoter, Shine-Dalgarno (SD) sequence, and signal peptide- and Protein A (SPA′)-encoding DNA in Spa′-pNK3260.
【Supplier来源】BioVector NTCC Inc.
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【Website网址】 http://www.biovector.net