pBG167 Recombinant Sindbis Virus Replicon Plasmid SINV BioVector NTCC质粒载体菌种细胞基因保藏中心

pBG167 Recombinant Sindbis Virus Replicon Plasmid SINV BioVector NTCC质粒载体菌种细胞基因保藏中心
Recombinant plasmid pBG167 expresses infectious Sindbis virus (SINV) and allows for fast and easy expression of your gene of interest.

Highlights:
Plasmid can be transfected into mammalian cells to produce infectious Sindbis virus
Unique XbaI site behind 3’ subgenomic promoter can be used to express genes of interest during infection

The entire Sindbis virus genome was inserted into the pcDNA3.1 Zeo+ expression vector such that the transcription start site of the CMV promoter overlaps with the first viral nucleotide of the viral genome. A Hepatitis D virus (HDV) ribozyme was added to the 3' end of the viral genomic 35bp polyA sequence. A unique XbaI restriction site 3' subgenomic promoter to allow genes of interest to be ligated into the viral genome that will be expressed only when the virus genome is replicated. Transfection of this construct into mammalian cells produces an infectious self-replicating Sindbis virus genome that can be modified to express genes of interest in vitro or in vivo.
Specifications



Product Type: Plasmid
Gene/insert name: Sindbis virus replicon
Organism: Sindbis Virus Strain TE3'2J (AR339)
Antibiotic Resistance: Amp
Grow in E. coli at 37 C: DH5-alpha or XL10-Gold E-coli
Cloning Site 5': MluI
Cloning Site 3': XhoI
Insert Size: 12,169 bp
Vector Backbone and Size: pcDNA3.1 Zeo+
High or low copy: High
Storage: Room Temperature (Dried), -20C (liquid)
Shipped: Room Temperature

【Supplier来源】BioVector NTCC Inc.
TEL：+86-010-53513060
【Website网址】 http://www.biovector.net