Detroit 532 Human Caucasian skin Down syndrome cell line BioVector NTCC质粒载体菌种细胞基因保藏中心

Detroit 532 Human Caucasian skin Down syndrome cell line BioVector NTCC质粒载体菌种细胞基因保藏中心
Cell Line Name: Detroit 532

Other Collection No.: CCL 54

Keywords: Human Caucasian, skin, Down syndrome
Cell Line Description: Derived from the foreskin of a 2 month old Caucasian with Down Syndrome.
Species: Human
Tissue of Origin: Skin

CellType: Fibroblast

Growth Mode: Adherent
DNA Profile: STR-PCR Data:
Amelogenin: X,Y
CSF1PO: 12
D13S317: 11,13
D16S539: 10,13
D5S818: 9,12
D7S820: 8
THO1: 7,9
TPOX: 8
vWA: 16,18
Karyotype: 2n = 46, A modal chromosome number 47 with trisomy for group 21 in 75% of the cells

Biosafety Information: Unless specified otherwise, at the European Collection of Authenticated Cell Cultures (ECACC) we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines. ACDP = Advisory Committee on Dangerous Pathogens (UK)
All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country.

ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

Hyperlinks to MSDS documents:
Frozen cell cultures Material Safety Data Sheet
Growing cell cultures Material Safety Data Sheet
Nucleic acids derived from cell cultures Material Safety Data Sheet


Subculture Routine: Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO2; 37°C. Finite lifespan, up to 30 passages expected.
Culture Medium: EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 0.1% Lactalbumin hydrolysate (LH) + 10% Foetal Bovine Serum (FBS).
Depositor: Obtained from ATCC
Originator: No
Country: USA

【Supplier来源】BioVector NTCC Inc.
TEL：+86-010-53513060
【Website网址】 http://www.biovector.net