SCC-090人口腔鳞癌细胞株SCC090，UPCI:SCC090 cell line BioVector NTCC质粒载体菌种细胞基因保藏中心
- 价 格：¥39865
- 货 号：SCC-090人口腔鳞癌细胞株SCC090，UPCI:SCC090 cell line
- 产 地：北京
- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作微信:1843439339 (QQ同号)
邮件：Biovector@163.com
手机：18901268599
地址：北京
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SCC-090人口腔鳞癌细胞株SCC090，UPCI:SCC090 cell line BioVector NTCC质粒载体菌种细胞基因保藏中心
Organism Homo sapiens, human
Tissue Derived from metastatic site: tongue
Cell Type squamous cell
Product Format frozen 1.0 mL
Morphology epithelial-like
Culture Properties adherent
Biosafety Level 2 [Cells contain human papillomavirus (HPV) DNA sequences]
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease squamous cell carcinoma; grade 3; pathological stage: T2N0
Age 46
Gender male
Ethnicity Caucasian
Applications This cell line represents an important resource for investigation into the tumor biology and the study of genetic abnormalities associated with the development and/or progression of HNSCC.
Derivation The surgically excised tumor was minced and distributed into flasks. When growth of fibroblasts obstructed the growth of the squamous cells, the fibroblasts were removed by trypsinization. The remaining squamous cells were grown and passaged.
Clinical Data
46 years
Caucasian
male
The donor smoked tobacco, drank alcoholic beverages, and died of his disease
Antigen Expression
CK 19+; Keratin IHC AE1+; Keratin IHC AE3+; CAM 5.2+
Oncogene amplification of chromosomal band 11q13, not expressed mutations of TP53 codon, not expressed
Comments UPCI:SCC090 is from a squamous cell carcinoma of the base of the tongue. These cells are positive for Human Papilloma Virus (HPV). They have no TP53 mutations as assayed by sequencing the 5-8 exons of TP53. UPCI:SCC090 showed no amplification of chromosomal band 11q13 using FISH.
The patient had a recurrence about 1 year later sited at the hypopharynx. It is ATCC® No. CRL-3240 UPCI:SCC152™.
These 2 HNSCC (head and neck squamous cell carcinoma) cell lines are a resource for studying the initiation, cancerization, prognosis, intervention and treatment of oral cancers.
Complete Growth Medium The base medium for this cell line is Eagle's Minimum Essential Medium . To make the complete growth medium, add the following components to the base medium:
additional 2mM L-Glutamine
fetal bovine serum (FBS) to a final concentration of 10%
Seeding Density 4 x 104 viable cells/cm2
Subculturing Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Remove and discard culture medium. Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) (ATCC® No. 30-2200) or 0.25% Trypsin – 0.53mM EDTA (ATCC® No. 30-2101) solution to remove all traces of serum which contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C.
Subcultivation Ratio: 1:2 to 1:4 is recommended.
Medium Renewal: 2 to 3 times a week
STR Profile Amelogenin: X,Y
CSF1PO: 11,12
D5S818: 11, 12
D7S820: 9, 10
D13S317: 11
D16S539: 12,13
TH01: 7
TPOX: 8
vWA: 17
【Supplier来源】BioVector NTCC Inc.
TEL：+86-010-53513060
【Website网址】 http://www.biovector.net
Organism Homo sapiens, human
Tissue Derived from metastatic site: tongue
Cell Type squamous cell
Product Format frozen 1.0 mL
Morphology epithelial-like
Culture Properties adherent
Biosafety Level 2 [Cells contain human papillomavirus (HPV) DNA sequences]
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease squamous cell carcinoma; grade 3; pathological stage: T2N0
Age 46
Gender male
Ethnicity Caucasian
Applications This cell line represents an important resource for investigation into the tumor biology and the study of genetic abnormalities associated with the development and/or progression of HNSCC.
Derivation The surgically excised tumor was minced and distributed into flasks. When growth of fibroblasts obstructed the growth of the squamous cells, the fibroblasts were removed by trypsinization. The remaining squamous cells were grown and passaged.
Clinical Data
46 years
Caucasian
male
The donor smoked tobacco, drank alcoholic beverages, and died of his disease
Antigen Expression
CK 19+; Keratin IHC AE1+; Keratin IHC AE3+; CAM 5.2+
Oncogene amplification of chromosomal band 11q13, not expressed mutations of TP53 codon, not expressed
Comments UPCI:SCC090 is from a squamous cell carcinoma of the base of the tongue. These cells are positive for Human Papilloma Virus (HPV). They have no TP53 mutations as assayed by sequencing the 5-8 exons of TP53. UPCI:SCC090 showed no amplification of chromosomal band 11q13 using FISH.
The patient had a recurrence about 1 year later sited at the hypopharynx. It is ATCC® No. CRL-3240 UPCI:SCC152™.
These 2 HNSCC (head and neck squamous cell carcinoma) cell lines are a resource for studying the initiation, cancerization, prognosis, intervention and treatment of oral cancers.
Complete Growth Medium The base medium for this cell line is Eagle's Minimum Essential Medium . To make the complete growth medium, add the following components to the base medium:
additional 2mM L-Glutamine
fetal bovine serum (FBS) to a final concentration of 10%
Seeding Density 4 x 104 viable cells/cm2
Subculturing Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Remove and discard culture medium. Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) (ATCC® No. 30-2200) or 0.25% Trypsin – 0.53mM EDTA (ATCC® No. 30-2101) solution to remove all traces of serum which contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C.
Subcultivation Ratio: 1:2 to 1:4 is recommended.
Medium Renewal: 2 to 3 times a week
STR Profile Amelogenin: X,Y
CSF1PO: 11,12
D5S818: 11, 12
D7S820: 9, 10
D13S317: 11
D16S539: 12,13
TH01: 7
TPOX: 8
vWA: 17
【Supplier来源】BioVector NTCC Inc.
TEL：+86-010-53513060
【Website网址】 http://www.biovector.net
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