FaDu human squamous cell carcinoma人鳞状上皮细胞癌细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

FaDu human squamous cell carcinoma人鳞状上皮细胞癌细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心
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Organism Homo sapiens, human
Tissue pharynx
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease squamous cell carcinoma
Age 56 years
Gender male
Ethnicity Caucasian
Applications This cell line is a suitable transfection host.
Karyotype (P16) hypodiploid to hypertriploid with modal number = 64
Derivation The FaDu line was established in 1968 from a punch biopsy of an hypopharyngeal tumor removed from a patient.
Clinical Data 56 years
Caucasian
male
The FaDu line was established in 1968 from a punch biopsy of an hypopharyngeal tumor removed from a patient.
Tumorigenic Yes
Effects Yes, in nude mice; forms well differentiated epidermoid carcinoma (grade I)
Comments The established line was found to contain bundles of tonofilaments in the cell cytoplasm and desmosomal regions were prominent at cell boundaries.
Complete Growth Medium The base medium for this cell line is Eagle's Minimum Essential Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation Culture medium, 95%; DMSO, 5%
Culture Conditions Temperature: 37°C
STR Profile Amelogenin: None detected
CSF1PO: 12
D13S317: 8, 9
D16S539: 11
D5S818: 12
D7S820: 11, 12
TH01: 8
TPOX: 11
vWA: 15, 17
Isoenzymes AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 2
Me-2, 2
PGM1, 2
PGM3, 1
Name of Depositor SR Rangan
Deposited As Homo sapiens
Year of Origin 1968
References Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871
Goodfellow M, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 77210034
Rangan SR. A new human cell line (FaDu) from a hypopharyngeal carcinoma. Cancer 29: 117-121, 1972. PubMed: 4332311
Faust JB, Meeker TC. Amplification and expression of the bcl-1 gene in human solid tumor cell lines. Cancer Res. 52: 2460-2463, 1992. PubMed: 1568216
Giard DJ, et al. In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors. J. Natl. Cancer Inst. 51: 1417-1423, 1973. PubMed: 4357758

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