E.coli::GFP荧光标记大肠杆菌菌株Escherichia coli GFP BioVector NTCC质粒载体菌种细胞基因保藏中心

E.coli::GFP荧光标记大肠杆菌菌株Escherichia coli GFP BioVector NTCC质粒载体菌种细胞基因保藏中心
Application Fluorescence detection
Fluorescence imaging
FACS
Cell infection assays
In vivo localization
Pathogen-host interactions assays
Biosafety Level 1

Antigenic Properties Serotype 06, Biotype 1

Antibiotic Resistance Ampicillin resistance gene (bla) encoded on a plasmid. Confers resistance to 100 µg/mL ampicillin.
Comments This clone was derived from E.coli 25922 and contains a multicopy vector encoding the green fluorescent protein GFPmut3. This gene is expressed under the control of the Placpromoter. This construct has been designed for Gram-negative bacteria fluorescence labeling.

Vector: pUCP18-MCSgfpmut3
Vector size: 5.492kb
Vector type: plasmid
Marker: bla (ampR)
Features:
GFPmut3: fluorescent maker under the control of Plac promoter
Origin of replication: ori from pRO1600
Unique restriction sites: BamHI, EcoRI, EcoRV, SmaI, XhoI.
Double restriction sites: HindIII, PstI, SalI, XbaI.

Medium Tryptic Soy Agar/Broth with 100 mcg/ml Ampicillin
Growth Conditions Temperature: 37°C
Atmosphere: Aerobic

References Choi KH, Schweizer HP. mini-Tn7 insertion in bacteria with single attTn7 sites: example Pseudomonas aeruginosa. Nat. Protoc. 1(1): 153-161, 2006. PubMed: 17406227

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