pBHG-fiber5/35腺病毒骨架质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

pBHG-fiber5/35腺病毒骨架质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心
Ad5/F35MaxTM vector packaging system based

on AdMax packaging system. Ad5/F35 is a chimeric adenovirus vector for the Ad5 virus fiber was modified into a type of the

35 adenovirus. In the genome only fiber gene is chimeric, and other genes still retain the characteristics of Ad5 vector. The

packaging system includes pBHG-fiber5/35 scaffold plasmid, shuttle plasmid and HEK293 cell. The mechanism is similar to

the AdMax system. After transfected into HEK-293 cells, the pBHG-fiber5/35 scaffold plasmid can match to the shuttle

plasmid carrying loxP site, and incur site directed recombination by Cre-loxP system, which can produce recombinant

adenovirus (Ad5/F35) with exogenous genes. The recombinant adenovirus obtained by this way is E1 defect replication

defective adenovirus, which can only express exogenous gene, but not be able to amplify itself in cell without E1 region. The

characteristic of Ad5/F35MaxTM adenovirus packaging system is to complete recombinant virus maturation by recombinant

enzyme in eukaryotic cell, which not only retains the advantages of quick virus maturation, good stability and high yield of

AdMax system, but also has advantages of wider infected cell range, higher infection efficiency (higher infection efficiency in

various hematopoietic cells and tumor cells in Ad5/F35 than that in common Ad5), more exogenous gene expression, and

efficient and stable packaging system.
【Supplier来源】BioVector NTCC Inc.
TEL：+86-010-53513060
【Website网址】 http://www.biovector.net