Propionibacterium acnes痤疮丙酸梭菌strain菌株菌种 BioVector NTCC质粒载体菌种细胞基因保藏中心

Propionibacterium acnes痤疮丙酸梭菌strain菌株菌种 BioVector NTCC质粒载体菌种细胞基因保藏中心
Propionibacterium acnes痤疮丙酸梭菌
NTCC-D1897

Name:
Cutibacterium acnes
Synonym(s):
Propionibacterium acnes (Gilchrist 1900) Douglas and Gunter 1946
Propionibacterium acnes subsp. elongatum Dekio et al. 2015
Propionibacterium acnes subsp. acnes (Gilchrist 1900) Dekio et al. 2015
Cutibacterium acnes subsp. elongatum (Dekio et al. 2015) Dekio et al. 2019
Cutibacterium acnes subsp. acnes (Gilchrist 1900) Nouioui et al. 2018 emend. Dekio et al. 2019
NTCC No.:
NTCC-D1897, Type strain
Isolated from:
acne lesion in human facial skin
Date of sampling:
before 04.09.1980
History:
<- A. Hayden (Corynebacterium acnes)
Genbank accession numbers:
16S rRNA gene: AB042288
whole genome shotgun sequence: AWZZ00000000

Cultivation conditions:
CHOPPED MEAT MEDIUM, anaerobic, 37°C
CHOPPED MEAT MEDIUM
Ground beef (fat free) 500.0 g
Distilled water 1000.0 ml
NaOH 1 N 25.0 ml
Use lean beef or horse meat. Remove fat and connective tissue before grinding. Mix
meat, water and NaOH, then boil for 15 min with stirring. Cool to room temperature,
skim fat off surface, and filter, retaining both meat particles and filtrate. To the filtrate
add water to a final volume of 1000 ml, and then add:
Casitone 30.0 g
Yeast extract 5.0 g
K HPO 5.0 g
Resazurin 1.0 mg
To make medium anoxic bring it to a boil, cool under 100% N gas atmosphere, add 0.5
g/l L-cysteine hydrochloride and adjust pH to 7.0. Dispense under 100% N gas
atmosphere by filling 7 ml medium into anoxic Hungate-type tubes (for strains
demanding meat particles put those first into the tube (use 1 part meat particles to 4 or
5 parts fluid)). Autoclave at 121C for 30 min. For agar slants use 15.0 g agar per
1000.0 ml medium.
In some cases (as indicated in the catalogue) the addition of Haemin and Vitamin K or
Vitamin K is necessary. Add to 1000 ml of medium after autoclaving:
Haemin solution (see below) 10.00 ml
Vitamin K or Vitamin K solution (see below) 10.00 ml
Haemin solution:
Dissolve 50 mg haemin in 1 ml 1 N NaOH; make up to 100 ml with distilled water and
filter sterilize. Store refrigerated.
Vitamin K solution:
Dissolve 0.1 ml of vitamin K in 20 ml 95% ethanol and filter sterilize. Store refrigerated
in a brown bottle.
Vitamin K solution:
Dissolve 5 mg/ml of vitamin K in 95% ethanol, dilute to 0.05 mg/ml in water and filter
sterilize. Store refrigerated in a brown bottle.

Incubation time: 1-2 days

Risk group:
2


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