RFP-GFP-LC3 adenovirus BioVector NTCC质粒载体菌种细胞基因保藏中心

RFP-GFP-LC3 adenovirus BioVector NTCC质粒载体菌种细胞基因保藏中心
Autophagy is a highly regulated homeostatic degradative process where cells destroy their own components via the lysosomal machinery and recycle them. This process plays both protective and deleterious roles in many diseases, including Alzheimer’s disease, aging, cancer, infection and Crohn’s disease. Elucidating the correlation between autophagy and apoptotic cell death has become the focus of a great deal of research. Members of the LC3 family play a key role in the maturation of the autophagosome. Lysosomal turnover of the autophagosomal marker LC3-II reflects starvation-induced autophagic activity, and detecting LC3 by immunoblotting or immunofluorescence has become a reliable method for monitoring autophagy and autophagy-related processes, including autophagic cell death.
BioVector has launched series of Adenovirus packaging service of autophage related biosensors, in which GFP and/or RFP tags are fused at the C-termini of the autophagosome marker LC3, allowing to detect the intensity of autophagy flux in real-time with more accuracy, clarity and intuitiveness. These biosensors provides an enhanced dissection of the maturation of the autophagosome to the autolysosome, which capitalizes on the pH difference between the acidic autolysosome and the neutral autophagosome. The acid-sensitive GFP will be degraded in autolysosome whereas the acid-insensitive RFP will not. Therefore, the change from autophagosome to autolysosome can be visualized by imaging the specific loss of the GFP fluorescence, leaving only red fluorescence. Taking advantage of RFP-GFP-LC3 and GFP-LC3 labeling system, BioVector has launched the production service of Adv-RFP-GFP-LC3 and Adv-GFP-LC3, which can be used to observe autophagy flux and monitor the intensity of autophagy flux in real-time in vivo or in vitro.

Quantity/Unit Vials
Form Frozen form
Sipping and Storage Guidelines Shipped by dry ice, stored at -80 ° C, effective for 1 year. Avoid repeatedly freezing and thawing
Titer > 1*10^10 PFU/ml
Advantages:
1. Autophagy flux detection in real time, quantitative.
2. High resolution, more accuracy and sensitivity than traditional approaches.
3. Broad range of host. Adv has the ability to infect dividing, quiescent cells, stem cells, and primary cells, allowing genetic materials to be delivered to a highly diverse range of cell types and tissues.
4. High infection efficiency. Almost 100% gene delivery in most cell types, completely surpassing other viral vector tools and liposome transfection.
5. Great packaging capacity, up to 8kb.
6. Without integration. No alteration to host genome.
7. High titer. Up to 1011 pfu/ml.

Figure 1
Adv-mRFP-GFP-LC3 indicates vector autophagy flux in Hela cells.

Figure 2
Confocal microscopic analysis of autophagosomes after amino acids deprivation (AAD)[1].
Quality control
Our optimized custom adenoviral vector production and strict quality control systems provide customers a high titer of functional recombinant adenoviral vectors. Viral titers are determined by TCID50 method, which is the most accurate way to measure the titer of adenovirus.

【Supplier来源】BioVector NTCC Inc.
TEL：+86-010-53513060
【Website网址】 http://www.biovector.net