MDCC-MSB1 cell line细胞株细胞系 BioVector NTCC质粒载体菌种细胞基因保藏中心

MDCC-MSB1 cell line细胞株细胞系 BioVector NTCC质粒载体菌种细胞基因保藏中心
Organism: Gallus gallus (chicken)
Morphology: Round cells
Cell type: Lymphoblast
Growth Properties: Suspension
References: Coleman RM et al. Independence of chicken major histocompatibility antigens and tumor
associated antigen on the surface of herpesvirus induced lymphoma cells. Infect Immun
29: 1067 72, 1980.
Culture Conditions and Handling
Culture Medium: RPMI 1640 supplemented with 2mM L glutamine and 10% fetal bovine serum
Subculturing: Establish new cultures at 3 x 10 viable cells/ml. Maintain the cell density between 1x10
and 1x10 cells/ml by transferring an appropriate amount of cell suspension into a new
cell culture flask refilled with fresh cell culture medium.
Seeding density: 1x10 cells/ml
Fluid Renewal: Renew medium by centrifuging the cell suspension, remove the medium and re suspend
in fresh medium every 2 to 3 days depending on cell density.
Doubling time: About 10 hours
Freeze Medium: CM 2 (CLS order number 800250, 50ml)
Freezing recovery: After thawing, allow the cells to recover from the freezing process for at least 24 hrs.
Sterility: Mycoplasma specific PCR: negative; Bacteria specific PCR: negative
Biosafety Level:
Safety precautions: If the cryovial is planned to be stored in liquid nitrogen and to be thawed in the future,
special safety precautions should be followed:
Protective gloves and clothing should be used and a facemask or safety goggles must be
worn when transferring frozen samples into or removing from the liquid nitrogen tank.
The removal of a cryovial from liquid nitrogen may result in the explosion of the frozen
vial creating flying fragments.
Caputo, J.L. Biosafety procedures in cell culture. J. Tissue Cult. Methods 11:223 227, 1988. ATCC
Quality Control Methods for Cell Lines, 2nd edition, 1992.
Special Features of the Cell Line
Viruses: SMRV: Negative, as confirmed by Real Time PCR
Recommendations for handling of cells growing in suspension following delivery
Cryopreserved cells The cells come deep frozen shipped on dry ice. Please make sure that the vial is still
frozen.
If immediate culturing is not intended, the cryovial(s) must be stored below 150°C after
arrival.
If immediate culturing is intended, please follow these instructions:
Quickly thaw by rapid agitation in a 37°C water bath within 40 60 seconds. The water
bath should have clean water containing an antimicrobial agent. As soon as the sample
has thawed, remove the cryovial from the water bath. Note: A small ice clump should still
remain and the vial should still be cold.
rom now on, all operations should be carried out under aseptic conditions.
Transfer the cryovial to a sterile flow cabinet and wipe with 70% alcohol. Carefully open
the vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of
ulture medium (room temperature). Resuspend the cells carefully. Centrifuge at 300xg
for 3 min and discard the supernatant. The centrifugation step may be omitted, but in this
case the remains of the freeze medium have to be removed 24 hours later.
Resuspend the cells carefully in 10ml fresh cell culture medium and transfer them into
one T25 cell culture flask. All further steps are described in the Subculture section.
Proliferating Cultures The cell culture flask, 1xT25, comes filled with cell culture medium.
Incubate at 37°C for a minimum of 24 hrs
Count the cells, spin down the cell suspension at 300x g for minutes to collect the cells.
Resuspend the cells in an appropriate amount of fresh cell culture medium and transfer
to new cell culture flasks.
Incubate at 37°C for a minimum of 24 hrs.
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