NCI-H1975 cell line细胞株细胞系H1975 BioVector NTCC质粒载体菌种细胞基因保藏中心
- 价 格：¥29865
- 货 号：NCI-H1975 cell line细胞株细胞系H1975
- 产 地：北京
- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作微信:1843439339 (QQ同号)
邮件：Biovector@163.com
手机：18901268599
地址：北京
- 已注册
NCI-H1975 cell line细胞株细胞系H1975 BioVector NTCC质粒载体菌种细胞基因保藏中心
Organism Homo sapiens, human
Tissue lung
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease adenocarcinoma; non-small cell lung cancer
Gender female
Derivation The line was established in July 1988. The tissue donor was a non-smoker.
Clinical Data female
The tissue donor was a non-smoker.
Complete Growth Medium The base medium for this cell line is RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing Remove medium, and rinse the monolayer with fresh 0.25% trypsin, 0.53 mM EDTA solution. Remove the trypsin, add fresh trypsin and let the culture sit at room temperature (or at 37°C) until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks. Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product.
Subcultivation Ratio: 1:3 to 1:6
Medium Renewal: two to three times weekly
Cryopreservation Freeze medium: culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions Temperature: 37°C
STR Profile Amelogenin: X
CSF1PO: 12
D13S317: 10,13
D16S539: 9,12
D5S818: 11,12
D7S820: 8,11
THO1: 7
TPOX: 8,11
vWA: 18
Year of Origin 1988
References NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.
Sordella R, et al. Gefitinib-sensitizing EGFR mutations in lung cancer activate anti-apoptotic pathways. Science 305: 1163-1167, 2004. PubMed: 15284455
【Supplier来源】BioVector NTCC Inc.
TEL：+86-010-53513060
【Website网址】 http://www.biovector.net
Organism Homo sapiens, human
Tissue lung
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease adenocarcinoma; non-small cell lung cancer
Gender female
Derivation The line was established in July 1988. The tissue donor was a non-smoker.
Clinical Data female
The tissue donor was a non-smoker.
Complete Growth Medium The base medium for this cell line is RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing Remove medium, and rinse the monolayer with fresh 0.25% trypsin, 0.53 mM EDTA solution. Remove the trypsin, add fresh trypsin and let the culture sit at room temperature (or at 37°C) until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks. Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product.
Subcultivation Ratio: 1:3 to 1:6
Medium Renewal: two to three times weekly
Cryopreservation Freeze medium: culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions Temperature: 37°C
STR Profile Amelogenin: X
CSF1PO: 12
D13S317: 10,13
D16S539: 9,12
D5S818: 11,12
D7S820: 8,11
THO1: 7
TPOX: 8,11
vWA: 18
Year of Origin 1988
References NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.
Sordella R, et al. Gefitinib-sensitizing EGFR mutations in lung cancer activate anti-apoptotic pathways. Science 305: 1163-1167, 2004. PubMed: 15284455
【Supplier来源】BioVector NTCC Inc.
TEL：+86-010-53513060
【Website网址】 http://www.biovector.net
- 公告/新闻
