EL4 cell line小鼠腹水淋巴瘤淋巴母细胞株细胞系 BioVector NTCC质粒载体菌种细胞基因保藏中心
- 价 格：¥39865
- 货 号：EL4 cell line小鼠腹水淋巴瘤淋巴母细胞株细胞系
- 产 地：北京
- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作微信:1843439339 (QQ同号)
邮件：Biovector@163.com
手机：18901268599
地址：北京
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EL4 cell line小鼠腹水淋巴瘤淋巴母细胞株细胞系 BioVector NTCC质粒载体菌种细胞基因保藏中心
Cell Line Name: EL4
Keywords: Mouse ascites lymphoma lymphoblast
Cell Line Description: Established from a lymphoma induced in a C57BL/6N mouse by 9,10-dimethyl-1,2-benzanthracene. The cells have been reported to produce low levels of type G (Gross) virus antigen (J Nat Cancer Inst 1972;48:265) H-2b, IL-4 and Thy-1.2 antigen. The cell line is resistant to cortisol and dexamethasone and is sensitive to PHA.
Species: Mouse
Tissue of Origin: blood
CellType: Lymphoblast
Growth Mode: Suspension
Karyotype: 2n = 39
Subculture Routine: When starting from a frozen ampoule the cryoprotectant should be removed. Add thawed cells to a conical based centrifuge tube e.g. 15ml tube, slowly add 4 ml of culture medium to the tube. Take a sample of the cell suspension, e.g. 100μl, to count cells. Centrifuge the cell suspension at low speed i.e. 100 - 150 x g for a maximum of 5 minutes. Remove medium and re-suspend the cell pellet at a density of 3 - 5 x 100,000 cells/ml in fresh medium. Incubate flask at 37°C; 5 - 7% CO2. Check daily. Subculture once exponential growth is established and maintain cultures between 3 - 9 x 100,000 cells/ml.
Culture Medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Country: UK
References: Br J Cancer 1950;4:372; Cancer Res 1965;25:813; J Immunol 1972;108:1146; J Nat Cancer Inst 1972;48:265
【Supplier来源】BioVector NTCC Inc.
TEL：+86-010-53513060
【Website网址】 http://www.biovector.net
Cell Line Name: EL4
Keywords: Mouse ascites lymphoma lymphoblast
Cell Line Description: Established from a lymphoma induced in a C57BL/6N mouse by 9,10-dimethyl-1,2-benzanthracene. The cells have been reported to produce low levels of type G (Gross) virus antigen (J Nat Cancer Inst 1972;48:265) H-2b, IL-4 and Thy-1.2 antigen. The cell line is resistant to cortisol and dexamethasone and is sensitive to PHA.
Species: Mouse
Tissue of Origin: blood
CellType: Lymphoblast
Growth Mode: Suspension
Karyotype: 2n = 39
Subculture Routine: When starting from a frozen ampoule the cryoprotectant should be removed. Add thawed cells to a conical based centrifuge tube e.g. 15ml tube, slowly add 4 ml of culture medium to the tube. Take a sample of the cell suspension, e.g. 100μl, to count cells. Centrifuge the cell suspension at low speed i.e. 100 - 150 x g for a maximum of 5 minutes. Remove medium and re-suspend the cell pellet at a density of 3 - 5 x 100,000 cells/ml in fresh medium. Incubate flask at 37°C; 5 - 7% CO2. Check daily. Subculture once exponential growth is established and maintain cultures between 3 - 9 x 100,000 cells/ml.
Culture Medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Country: UK
References: Br J Cancer 1950;4:372; Cancer Res 1965;25:813; J Immunol 1972;108:1146; J Nat Cancer Inst 1972;48:265
【Supplier来源】BioVector NTCC Inc.
TEL：+86-010-53513060
【Website网址】 http://www.biovector.net
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