GM2163 E.coli大肠杆菌Dam,Dcm缺陷型菌株,感受态细胞 BioVector NTCC质粒载体菌种细胞基因保藏中心
- 价 格:¥39865
- 货 号:GM2163 E.coli大肠杆菌Dam,Dcm缺陷型菌株,感受态细胞
- 产 地:北京
- BioVector NTCC典型培养物保藏中心
- 联系人:Dr.Xu, Biovector NTCC Inc.
电话:400-800-2947 工作微信:1843439339 (QQ同号)
邮件:Biovector@163.com
手机:18901268599
地址:北京
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GM2163 E.coli大肠杆菌Dam,Dcm缺陷型菌株,感受态细胞 BioVector NTCC质粒载体菌种细胞基因保藏中心
GM2163 is an E. coli K12 strain, which is deficient in both dam and dcm genes thus is suitable for the propagation of plasmid or cloned DNA to be cut with Dam or Dcm-sensitive restriction enzymes. This strain is not recommended as a host for primary cloning/ligation since the dam mutation can result in higher mutation rates and a reduction in the transformation efficiency.
Genotype:
F–ara–14 leuB6 fhuA31 lacY1 tsx78 glnV44 galK2 galT22 mcrA dcm–6 hisG4 rfbD1 rpsL136 dam13::Tn9 xylA5 mtl–1 thi-1 mcrB1 hsdR2
Features
Chloramphenicol resistant
Not suitable for blue/white screening
Not recommended as a host for primary cloning/ligation
Applications
Propagation of plasmid-free of Dam and Dcm methylations
【Supplier来源】BioVector NTCC Inc.
TEL:+86-010-53513060
【Website网址】 http://www.biovector.net
GM2163 is an E. coli K12 strain, which is deficient in both dam and dcm genes thus is suitable for the propagation of plasmid or cloned DNA to be cut with Dam or Dcm-sensitive restriction enzymes. This strain is not recommended as a host for primary cloning/ligation since the dam mutation can result in higher mutation rates and a reduction in the transformation efficiency.
Genotype:
F–ara–14 leuB6 fhuA31 lacY1 tsx78 glnV44 galK2 galT22 mcrA dcm–6 hisG4 rfbD1 rpsL136 dam13::Tn9 xylA5 mtl–1 thi-1 mcrB1 hsdR2
Features
Chloramphenicol resistant
Not suitable for blue/white screening
Not recommended as a host for primary cloning/ligation
Applications
Propagation of plasmid-free of Dam and Dcm methylations
【Supplier来源】BioVector NTCC Inc.
TEL:+86-010-53513060
【Website网址】 http://www.biovector.net
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