dadA E.coli mutant大肠杆菌基因缺失菌株-BioVector NTCC保藏中心

dadA E.coli mutant大肠杆菌基因缺失菌株-BioVector NTCC保藏中心
Gene Symbol突变基因名称：dadA
Mnemonic:_D-amino acid dehydrogenase
Synonyms别称：dadR
Gene product基因产物：_D-amino acid dehydrogenase subunit
Selectable Phenotype筛选表型：form white colonies on tetrazolium agar plates (Bochner, and savageau 1977) supplemented with _L- or _D-alanine
Culture Condition培养条件：rich medium made from dry broth, from Biomed (Warszawa, Poland), of Difco (Detroit, USA)
Other Phenotypes其他表型：•resistant to beta-chloro-_D-alanine (The inhibitory effect was examined by applying 0.1 micro-mole aliquots of beta-chloro-_D-alanine to filter paper discs on minimal agar plates overlayed with soft agar containing wild-type or mutant cells. The analog produced inhibition zones, with a diameter of about 3.5 cm, on wild-types strains only. )
Notes：•in trp^- or other amino acid auxotroph: grow on _D-tryptophan (20 μg/ml) or other _D-amino acid (Proc. Natl. Acad. Sci. USA. 1971. 68: 2484-2487)
Reference参考文献：Wild, J. & T. Klopotowski
Mol. Gen. Genet. 1981. 181: 373-378

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