Karpase 299 cell line细胞株细胞系 BioVector NTCC质粒载体菌种细胞基因保藏中心
- 价 格：¥19865
- 货 号：Karpase 299 cell line细胞株细胞系
- 产 地：北京
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- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作微信:1843439339 (QQ同号)
邮件：Biovector@163.com
手机：18901268599
地址：北京
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Karpase 299 cell line细胞株细胞系 BioVector NTCC质粒载体菌种细胞基因保藏中心
Cell Line Name: KARPAS 299
Keywords: Human Non-Hodgkin's Ki-positive Large Cell Lymphoma
Cell Line Description: A human T cell lymphoma cell line established from the peripheral blood of a 25-year-old man with T cell non-Hodgkin's lymphoma in 1986; now classed as CD30+ anaplastic large cell lymphoma (ALCL); cells carry the NPM-ALK fusion gene.
Species: Human
Tissue of Origin: Lymphoma
CellType: Lymphoblastoid
Growth Mode: Suspension
DNA Profile: STR-PCR Data:
Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 8,12
D16S539: 12
D5S818: 11,12
D7S820: 10,11
THO1: 7,8.3
TPOX: 8
vWA: 17,19
Karyotype: Hypodiploid
Subculture Routine: Maintain cultures between 0.5 - 2 x1000,000 cells/ml; 5% CO2; 37°C. Doubling time approximately 30 hours. Split saturated culture 1:3 every 2-3 days. Round to polygonal cells growing singly or in small clusters in suspension.
Culture Medium: RPMI 1640 + 2mM Glutamine + 20% Foetal Bovine Serum (FBS). For slower growth use 10% or 5% FBS.
Originator: Yes
Country: UK
References: Fischer P, Nacheva E, Mason DY, Sherrington PD, Hoyle C, Hayhoe FG, Karpas A. 1988 A Ki-1 (CD30)-positive human cell line (Karpas 299) established from a high-grade non-Hodgkin's lymphoma, showing a 2; 5 translocation and rearrangement of the T-cell receptor beta-chain gene. Blood. 72(1):234-40 PMID: 3260522.
【Supplier来源】BioVector NTCC Inc.
TEL：+86-010-53513060
【Website网址】 http://www.biovector.net
Cell Line Name: KARPAS 299
Keywords: Human Non-Hodgkin's Ki-positive Large Cell Lymphoma
Cell Line Description: A human T cell lymphoma cell line established from the peripheral blood of a 25-year-old man with T cell non-Hodgkin's lymphoma in 1986; now classed as CD30+ anaplastic large cell lymphoma (ALCL); cells carry the NPM-ALK fusion gene.
Species: Human
Tissue of Origin: Lymphoma
CellType: Lymphoblastoid
Growth Mode: Suspension
DNA Profile: STR-PCR Data:
Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 8,12
D16S539: 12
D5S818: 11,12
D7S820: 10,11
THO1: 7,8.3
TPOX: 8
vWA: 17,19
Karyotype: Hypodiploid
Subculture Routine: Maintain cultures between 0.5 - 2 x1000,000 cells/ml; 5% CO2; 37°C. Doubling time approximately 30 hours. Split saturated culture 1:3 every 2-3 days. Round to polygonal cells growing singly or in small clusters in suspension.
Culture Medium: RPMI 1640 + 2mM Glutamine + 20% Foetal Bovine Serum (FBS). For slower growth use 10% or 5% FBS.
Originator: Yes
Country: UK
References: Fischer P, Nacheva E, Mason DY, Sherrington PD, Hoyle C, Hayhoe FG, Karpas A. 1988 A Ki-1 (CD30)-positive human cell line (Karpas 299) established from a high-grade non-Hodgkin's lymphoma, showing a 2; 5 translocation and rearrangement of the T-cell receptor beta-chain gene. Blood. 72(1):234-40 PMID: 3260522.
【Supplier来源】BioVector NTCC Inc.
TEL：+86-010-53513060
【Website网址】 http://www.biovector.net
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