AGE1.HN cell line悬浮高效表达细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心
- 价 格：¥3989860
- 货 号：AGE1.HN cell line悬浮高效表达细胞株
- 产 地：北京
- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作微信:1843439339 (QQ同号)
邮件：Biovector@163.com
手机：18901268599
地址：北京
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AGE1.HN cell line悬浮高效表达细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心
The AGE1.HN cell line was developed from primary cells from a human brain tissue sample. The primary cells were immortalized with an expression plasmid containing the adenoviral E1 A and B genes of human adenovirus type 5 driven by the human pGK and the endogenous E1B promoter, respectively. While the E1A induces progression of the cell cycle, the E1B proteins prevent apoptosis caused by E1A through interaction with P53 and bax. The cell line was further modified to express the structural and regulatory protein pIX from human adenovirus type 5. This modification changes cell metabolism, enhances productivity for secreted proteins and modulates susceptibility to a variety of viruses. The AGE1.HN cells express marker genes representative for cells of neuronal lineage and lack expression of glial markers. The cell line was adapted to robust growth in suspension in both serum/protein-free and chemically defined culture media and has been proven to grow efficiently in shaker, spinner, roller and stirred tank bioreactors. The entire development work for the generation of the AGE1.HN line was carried out in a dedicated cell culture suite and the complete development history was recorded. The documentation for all media and supplements used in the AGE1.HN cell line development is in compliance with the stipulations of EMEA 410/01.
Cell line name AGE1.HN
Transfected with: Adenovirus 5 pIX.
Transformant: Adenovirus 5 [E1A/E1B].
Species of origin Homo sapiens (Human)
Category Transformed cell line
The cells were cultured in baffled shake flasks at 37 °C on a shaker (2 inches orbit, ES-X, Kühner, Basel, Switzerland) enclosed in a 5% CO2 supplied, humidified (80%) incubator. The pre-culture was carried out in a 250 ml baffled shake flask in 42-Max-UB-medium which is serum-free and was especially developed for the AGE1.HN cell line. The cells were centrifuged (800 l min−1, 7 min, 22 °C) and the supernatant discarded. The pellet was resuspended in 42-Max-UB-medium without glucose and glutamine. Four 125-ml baffled shake flasks were inoculated yielding an initial cell density of about 4 × 105 cells ml−1. Glucose and glutamine were finally added. The final culture volume was 54 ml. Samples (1.6 ml) were taken every day. 500 μl were directly used for cell counting. The rest was centrifuged (1,500 l min−1, 5 min, 22 °C), the supernatant transferred into fresh tubes and frozen (−20 °C). Dissolved oxygen was always above 80% in the applied setup which was determined in an additional cultivation using the OXY-4 mini system.
【Supplier来源】BioVector NTCC Inc.
TEL：+86-010-53513060
【Website网址】 http://www.biovector.net
The AGE1.HN cell line was developed from primary cells from a human brain tissue sample. The primary cells were immortalized with an expression plasmid containing the adenoviral E1 A and B genes of human adenovirus type 5 driven by the human pGK and the endogenous E1B promoter, respectively. While the E1A induces progression of the cell cycle, the E1B proteins prevent apoptosis caused by E1A through interaction with P53 and bax. The cell line was further modified to express the structural and regulatory protein pIX from human adenovirus type 5. This modification changes cell metabolism, enhances productivity for secreted proteins and modulates susceptibility to a variety of viruses. The AGE1.HN cells express marker genes representative for cells of neuronal lineage and lack expression of glial markers. The cell line was adapted to robust growth in suspension in both serum/protein-free and chemically defined culture media and has been proven to grow efficiently in shaker, spinner, roller and stirred tank bioreactors. The entire development work for the generation of the AGE1.HN line was carried out in a dedicated cell culture suite and the complete development history was recorded. The documentation for all media and supplements used in the AGE1.HN cell line development is in compliance with the stipulations of EMEA 410/01.
Cell line name AGE1.HN
Transfected with: Adenovirus 5 pIX.
Transformant: Adenovirus 5 [E1A/E1B].
Species of origin Homo sapiens (Human)
Category Transformed cell line
The cells were cultured in baffled shake flasks at 37 °C on a shaker (2 inches orbit, ES-X, Kühner, Basel, Switzerland) enclosed in a 5% CO2 supplied, humidified (80%) incubator. The pre-culture was carried out in a 250 ml baffled shake flask in 42-Max-UB-medium which is serum-free and was especially developed for the AGE1.HN cell line. The cells were centrifuged (800 l min−1, 7 min, 22 °C) and the supernatant discarded. The pellet was resuspended in 42-Max-UB-medium without glucose and glutamine. Four 125-ml baffled shake flasks were inoculated yielding an initial cell density of about 4 × 105 cells ml−1. Glucose and glutamine were finally added. The final culture volume was 54 ml. Samples (1.6 ml) were taken every day. 500 μl were directly used for cell counting. The rest was centrifuged (1,500 l min−1, 5 min, 22 °C), the supernatant transferred into fresh tubes and frozen (−20 °C). Dissolved oxygen was always above 80% in the applied setup which was determined in an additional cultivation using the OXY-4 mini system.
【Supplier来源】BioVector NTCC Inc.
TEL：+86-010-53513060
【Website网址】 http://www.biovector.net
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