pMP720，pMP749，pMP793，pMP672转座子Tn7质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

pMP720，pMP749，pMP793，pMP672转座子Tn7质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心
Maps of the Tn7 system vectors. (a) The helper plasmid pMP720 is an unstable E. coli–F. tularensis shuttle vector that contains the Francisella blaB promoter driving
the site- and orientation-specific transposase complex tnsABCD for integrating (b) the mini-Tn7 pMP749, which contains the transposon end Tn7L, a multiple cloning site,
two terminators (T1 and T0) to limit readthrough from the glmS promoter on insertion, PgroESL–aphA-1 (conferring resistance to kanamycin flanked by the cd-sites for resolution
of the cassette) and the other transposon end Tn7R, or (c) pMP793, which has the Francisella rpsL promoter driving gfp expression in the multiple cloning site of pMP749. (d)
The resolvase plasmid pMP672 is an unstable E. coli–F. tularensis shuttle vector which has tnpR, encoding cd-resolvase, driven by the blaB promoter for resolution of the
kanamycin cassette after insertion into the Francisella genome.
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