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Rosetta (DE3) strain菌株菌种/感受态细胞 BioVector NTCC质粒载体菌种细胞基因保藏中心

  • 价  格:¥9865
  • 货  号:Rosetta (DE3) strain菌株菌种/感受态细胞
  • 产  地:北京
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Rosetta (DE3) strain菌株菌种/感受态细胞 BioVector NTCC质粒载体菌种细胞基因保藏中心
Rosetta™ host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli. These strains supply tRNAs for AGG, AGA, AUA, CUA, CCC, GGA codons on a compatible chloramphenicol-resistant plasmid. Thus the Rosetta strains provide for “universal” translation which is otherwise limited by the codon usage of E. coli. The tRNA genes are driven by their native promoters.

DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.

Genotype: F-ompT hsdSB(rB- mB-) gal dcm (DE3) pRARE (CamR)

【Supplier来源】BioVector NTCC Inc.
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