pProLabel-T哺乳动物细胞表达质粒Co-IP系统对照质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

pProLabel-T哺乳动物细胞表达质粒Co-IP系统对照质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心
pProLabel-T Vector is a mammalian expression vector encoding a fusion of the SV40 large T
antigen at the C-terminus of the ProLabel tag (~6 kDa). Because ProLabel is the a fragment of the split b-galactosidase
enzyme, the ProLabel-T fusion protein alone has no enzymatic activity. However, the ProLabel-T
fusion can recombine with the Ω fragment of b-galactosidase to reconstitute an active enzyme. The SV40
polyadenylation signals downstream of the ProLabel-SV40 T gene direct proper processing of the 3’end of
mRNA. The vector backbone also contains an SV40 origin for replication in any mammalian cell line that
expresses the SV40 T antigen. A neomycin resistance cassette (Neor
), consisting of the SV40 early promoter,
the neomycin/kanamycin resistance gene of Tn5, and polyadenylation signals from the herpes simplex virus
thymidine kinase (HSV-TK) gene, allows stably transfected eukaryotic cells to be selected using G418. A
bacterial promoter upstream of this cassette drives expression of the gene encoding kanamycin resistance
in E. coli. The pProLabel-C vector backbone also provides a pUC origin of replication for propagation in E.
coli and an f1 origin for single-stranded DNA production.
【Supplier来源】BioVector NTCC Inc.
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【Website网址】 http://www.biovector.net