pProLabel-C哺乳动物细胞Co-IP系统表达质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

pProLabel-C哺乳动物细胞Co-IP系统表达质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心
pProLabel-C Vector is a linearized mammalian expression vector encoding the ProLabel tag
(~6 kDa). The coding sequence of the ProLabel tag is comprised of the a fragment of b-galactosidase
which, when combined with the Ω fragment of b-galactosidase, produces an active
b-galactosidase enzyme. The MCS of pProLabel-C has been linearized with SalI/BamHI and genes cloned into
the vector at this site will be expressed as fusions to the C-terminus of the ProLabel tag if they are in the
same reading frame as the ProLabel and there are no intervening stop codons. SV40 polyadenylation signals
downstream of the ProLabel gene direct proper processing of the 3’ end of mRNA. The vector backbone
also contains an SV40 origin for replication in any mammalian cell line that expresses the SV40 T antigen.
A neomycin resistance cassette (Neor
), consisting of the SV40 early promoter, the neomycin/kanamycin resistance
gene of Tn5, and polyadenylation signals from the herpes simplex virus thymidine kinase (HSV-TK)
gene, allows stably transfected eukaryotic cells to be selected using G418. A bacterial promoter upstream
of this cassette drives expression of the gene encoding kanamycin resistance in E. coli. The pProLabel-C
vector backbone also provides a pUC origin of replication for propagation in E. coli and an f1 origin for
single-stranded DNA production.
【Supplier来源】BioVector NTCC Inc.
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【Website网址】 http://www.biovector.net