pAcGFP-C荧光表达载体Co-IP系统质粒 BioVector NTCC质粒载体菌种细胞基因保藏中心

pAcGFP-C荧光表达载体Co-IP系统质粒 BioVector NTCC质粒载体菌种细胞基因保藏中心
Description. pAcGFP1-C Vector is a mammalian expression vector that encodes a green fluorescent protein
(GFP) from Aequorea coerulescens. The fluorescent protein coding sequence in this construct has been
human-codon-optimized for efficient expression and enhanced brightness. AcGFP1 protein has an excitation
maximum at 475 nm and an emission maximum at 505 nm. Sequences flanking AcGFP1 have been
converted to a Kozak consensus translation initiation site (1) to further increase translation efficiency in
eukaryotic cells. A gene of interest can be added in-frame downstream of the AcGFP1 coding sequence and
expressed as a fusion protein to the C-terminus of AcGFP1. SV40 polyadenylation signals downstream of the
AcGFP1 gene direct proper processing of the 3’end of the mRNA transcript. In addition, the vector also contains
a SV40 origin of replication in mammalian cells expressing the SV40 T-antigen. A neomycin resistance
cassette (Neor
) containing an SV40 early promoter, the neomycin/kanamycin resistance gene of Tn5, and
polyadenylation signals from the Herpes simplex virus thymidine kinase (HSV TK) gene, allows selection of
stable transformants in eukaryotic cells using G418. A bacterial promoter upstream of the gene expresses
kanamycin resistance in E. coli. pAcGFP-C Vector also provides a pUC origin of replication for propagation in
E. coli and an f1 origin for single-stranded DNA production.
【Supplier来源】BioVector NTCC Inc.
TEL：+86-010-53513060
【Website网址】 http://www.biovector.net