MOLP-8 cell line细胞株细胞系 BioVector NTCC质粒载体菌种细胞基因保藏中心

MOLP-8 cell line细胞株细胞系 BioVector NTCC质粒载体菌种细胞基因保藏中心
Cell line:
MOLP-8
DSMZ no.:
ACC 569
Species:
human (Homo sapiens)
Cell type:
multiple myeloma
Origin:
established from the peripheral blood of a 52-year-old Japanese man with multiple myeloma (stage IIIA, type IgD lambda) in 2002
Reference(s):
14748
Biosafety level:
1
Permissions and restrictions:
A

DSMZ Cell Culture Data:
Morphology:
single round cells in suspension, some cells loosely adherent; image; image
Medium:
80% RPMI 1640 + 20% h.i. FBS
Subculture:
seed out at ca. 0.5 x 106 cells/ml; maintain at 0.5-1.5 x 106 cells/ml; split saturated culture 1:2 to 1:3 every 2-3 days; max. density at about 1.5 x 106 cells/ml; cells should be grown in 24- or 12-well-plates; immediately after thawing viability may drop to 50%, but cells recover within a few days
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 40 hours
Harvest:
cell harvest of ca. 1 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO

DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
CD3 -, CD10 +, CD13 -, CD19 -, CD20 -, CD34 -, CD37 -, CD38 +, cyCD79a -, CD80 -, CD138 +, HLA-DR -, sm/cyIgG -, sm/cyIgM -, sm/cykappa -, sm/cylambda +; image
Fingerprint:
fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile
Species:
confirmed as human by cytogenetics and species PCR
Cytogenetics:
human flat-moded near-diploid karyotype - 40-48<2n>X, -Y, -15, +1-2mar, t(3;8)(p22;q22), der(4)t(4;15)(q32;q21), inv(8)(q21q24), der(11)t(?4 ;11)(q31;q13), t(11;14)(q13;q32), t(16;18)(p13;q21), der(17)t(11;17)(q13;p13) - carries t(11;14) effecting IGH-CCND1 juxtaposition with additional MYC rearrangement present in inv(8) - matches published karyotype
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -
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