ST cell line猪小肠成纤维细胞 BioVector NTCC质粒载体菌种细胞基因保藏中心

ST cell line猪小肠成纤维细胞 BioVector NTCC质粒载体菌种细胞基因保藏中心
ST cell line
Cat No.: NTCC-A1746
Organism Sus scrofa, pig
Tissue testis
Product Format frozen
Morphology fibroblast
Culture
Properties adherent
Biosafety Level 1
Disease Normal
Age 80 to 90 days gestation
Gender male
Applications This line is useful for the propagation, assay and isolation of PPV, TGE and swine enteroviruses.
Karyotype diploid
Biovector NTCC, Inc
No.19,Xizhimen,Beijing,China
http://www.Biovector.net
Tel:010-53513060. 400-800-2947
Images
Clinical Data male
80 to 90 days gestation
Complete
Growth Medium
The base medium for this cell line is Eagle's Minimum Essential Medium. To make the complete growth
medium, add the following components to the base medium: fetal bovine serum to a final concentration
of 10%.
Subculturing Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed
proportionally for culture vessels of other sizes.
1. Remove and discard culture medium.
2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all
traces of serum that contains trypsin inhibitor.
3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted
microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the
cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
5. Add appropriate aliquots of the cell suspension to new culture vessels.
6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture
Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
Biovector NTCC, Inc
No.19,Xizhimen,Beijing,China
http://www.Biovector.net
Tel:010-53513060. 400-800-2947
References McClurkin AW, Norman JO. Studies on transmissible gastroenteritis of swine. II. Selected characteristics
of a cytopathogenic virus common to five isolates from transmissible gastroenteritis. Can. J. Comp. Med.
Vet. Sci. 30: 190-198, 1966. PubMed: 4224292
【Supplier来源】BioVector NTCC Inc.
TEL：+86-010-53513060
【Website网址】 http://www.biovector.net