MDBK cell line细胞株细胞系 BioVector NTCC质粒载体菌种细胞基因保藏中心
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- 货 号：MDBK cell line细胞株细胞系
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MDBK cell line细胞株细胞系 BioVector NTCC质粒载体菌种细胞基因保藏中心
Cell Line Name: MDBK
Keywords: Bovine kidney
Cell Line Description: Derived from the kidney of an apparently normal, adult Bos taurus. Also known as MDBK (NBL-1).
The Bovine Viral Diarrhoea Virus (BVDV) status of this cell line was previously described as negative. Results of recent multiple molecular tests for BVDV that were undertaken by two independent testing laboratories were equivocal. This means that we cannot confirm the BVDV status of this cell line.
Species: Bovine
Tissue of Origin: kidney
CellType: Epithelial-like
Growth Mode: Adherent
Karyotype: Modal no. 51; hypodiploid
Biosafety Information: 2
Subculture Routine: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Culture Medium: EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS) or 10% Horse Serum (HS).
Originator: No
Country: USA
References: Proc. Soc. Exp. Biol. Med 1958;98:574; J Nat Cancer Inst 1986;76:87
【Supplier来源】BioVector NTCC Inc.
TEL：+86-010-53513060
【Website网址】 http://www.biovector.net
Cell Line Name: MDBK
Keywords: Bovine kidney
Cell Line Description: Derived from the kidney of an apparently normal, adult Bos taurus. Also known as MDBK (NBL-1).
The Bovine Viral Diarrhoea Virus (BVDV) status of this cell line was previously described as negative. Results of recent multiple molecular tests for BVDV that were undertaken by two independent testing laboratories were equivocal. This means that we cannot confirm the BVDV status of this cell line.
Species: Bovine
Tissue of Origin: kidney
CellType: Epithelial-like
Growth Mode: Adherent
Karyotype: Modal no. 51; hypodiploid
Biosafety Information: 2
Subculture Routine: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Culture Medium: EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS) or 10% Horse Serum (HS).
Originator: No
Country: USA
References: Proc. Soc. Exp. Biol. Med 1958;98:574; J Nat Cancer Inst 1986;76:87
【Supplier来源】BioVector NTCC Inc.
TEL：+86-010-53513060
【Website网址】 http://www.biovector.net
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