Streptococcus saliva唾液链球菌Streptococcus salivarius BioVector NTCC质粒载体菌种细胞基因保藏中心

Streptococcus saliva唾液链球菌Streptococcus salivarius BioVector NTCC质粒载体菌种细胞基因保藏中心
Streptococcus salivarius唾液链球菌
NTCC-D20067

Name:
Streptococcus salivarius Andrewes and Horder 1906
Synonym(s):
Streptococcus salivarius subsp. salivarius (Andrewes and Horder 1906) Farrow and Collins 1984
No.:
D20067
Strain designation:
21367
Biotype:
II
Isolated from:
dental caries
Country:
country of origin unknown
Date of sampling:
before 22.08.1990
History:
<- Kiel, 21367 (Streptococcus salivarius biotype II)
Cultivation conditions:
Medium 92 , microaerophilic, 37°C
or
Medium 693 , microaerophilic, 37°C

Incubation time: 1-2 days

Please follow special instructions: 'Cultivation of Streptococci and Enterococci'

Medium 92. TRYPTICASE SOY YEAST EXTRACT MEDIUM
Trypticase soy broth 30.0 g
Yeast extract 3.0 g
Agar, if necessary 15.0 g
Distilled water 1000.0 ml
Adjust pH to 7.0 - 7.2


Medium 693. COLUMBIA BLOOD MEDIUM
Columbia agar base supplemented with 5% defibrinated sheep blood. Rehydrate pellets
of freeze-dried ampoules in liquid medium 545, 215 or 1 (see below).
ATTENTION: organisms, for which only M 693 is mentioned in the catalogue entry, do
not grow in one of the media listed below! Those are meant to rehydrate the freezedried
pellets, not for cultivation.
545. TRYPTONE SOYA BROTH (TSB)
Peptone from casein 17.0 g
Peptone from soymeal 3.0 g
D(+)-Glucose 2.5 g
NaCl 5.0 g
K2HPO4 2.5 g
Distilled water 1000.0 ml
Adjust pH to 7.3.
The same as Caso Bouillon (Merck or Oxoid, Germany).
215. BHI MEDIUM
Brain Heart Infusion (Difco) 37.0 g
Distilled water 1000.0 ml
1. NUTRIENT BROTH
Peptone 5.0 g
Meat extract 3.0 g
Distilled water 1000.0 ml
Adjust pH to 7.0.


Summary and
additional information:
<- Kiel, 21367 (Streptococcus salivarius biotype II). Dental caries; country of origin unknown; sampling date: before 22.08.1990. Biotype II. Murein: A11.24 (405). (Medium 92, 37°C, microaerophilic or Medium 693, 37°C, microaerophilic).
Risk group:
2


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