pNKY51酵母表达载体质粒 BioVector NTCC质粒载体菌种细胞基因保藏中心

pNKY51酵母表达载体质粒 BioVector NTCC质粒载体菌种细胞基因保藏中心
pNKY51 (hisG direct repeats separated by URA3): pNKY5 1 was built in four steps. (1) The backbone, pNKY3 was made by deleting the 2micron DNA from YEP24 with EcoRI and by inserting a BglII linker at the remaining EcoRI site (EcoR1 site is regenerated). (2) A 1.1kb BglII-BamHI fragment of pNK294 bearing Salmonella hisG DNA was inserted into the BglII site of pNKY3 to form pNKY49. (3) The same 1.1-kb hisG fragment as in (2) was inserted at the BamHI site of pNKY49 to form pNKY50. (4) The EcoRI site at the 5’ end of URA3 in pNKY50 was destroyed by fill in and ligation reactions. The resulting plasmid, pNKY51, contains the 3.8 kb hisG-URA3-hisG fragment that can be gel isolated by a BglII and BamHI digest.

Sequence deposited by author is for the hisG-URA3-hisG insert.
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