SC-1 cell line细胞株细胞系 BioVector NTCC质粒载体菌种细胞基因保藏中心

SC-1 cell line细胞株细胞系 BioVector NTCC质粒载体菌种细胞基因保藏中心
SC-1
ACC 558

Cell line:
SC-1

No.:
ACC 558
Species:
human (Homo sapiens)
Cell type:
B cell lymphoma
Origin:
established from the ascitic fluid of a 67-year-old man at diagnosis of B cell lymphoma (B-NHL, follicular lymphoma, small cleaved cell type) in 1977

Biosafety level:
1
Permissions and restrictions:
A

Cell Culture Data:
Morphology:
lymphoid cells growing singly or in small clumps in suspension;


Medium:
80-90% RPMI 1640 + 10-20% h.i. FBS
Subculture:
seed out at ca. 1.5 x 106 cells/ml; split saturated culture 1:2 to 1:4 every 3-5 days; maintain at 1-2 x 106 cells/ml; max. density at ca. 3.5 x 106 cells/ml
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 30 hours
Harvest:
cell harvest of ca. 3 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO

Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
CD3 -, CD10 +, CD13 -, CD19 +, CD34 -, CD37 +, CD38 +, cyCD79a (+), CD80 +, CD138 +, HLA-DR +, sm/cyIgG +, sm/cyIgM -, sm/cykappa -, sm/cy lambda -; image
Fingerprint:
fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile
Species:
confirmed as human by PCR speciation and cytogenetics
Cytogenetics:
human hyperdiploid karyotype with 4% polyploidy - 47(43-48)<2n>XY, +7, -17, +mar, add(3)(q27), t(8;14;18)(q24;q32;q21), add(14)(q32) - carries 3-way t(8,14,18) effecting genomic co-amplification of BCL2 and MYC - resembles published karyotype
Molec. Genetics:
expression of fusion gene BCL2-IGH confirmed by RT-PCR
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -


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