L-428 cell line细胞株细胞系 BioVector NTCC质粒载体菌种细胞基因保藏中心

L-428 cell line细胞株细胞系 BioVector NTCC质粒载体菌种细胞基因保藏中心
Cell line:
L-428
DSMZ no.:
ACC 197
Species:
human (Homo sapiens)
Cell type:
Hodgkin lymphoma
Origin:
established from the pleural effusion of a 37-year-old woman with Hodgkin lymphoma (stage IVB, nodular sclerosis, refractory, terminal) in 1978
Biosafety level:
1

DSMZ Cell Culture Data:
Morphology:
single cells in suspension or as clusters; polymorph cells, some cells have "spikes", 1-5% giant multinucleated cells;

Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out at ca. 0.5 x 106 cells/ml; after an initial slow start cells grow quickly; maintain at 0.2-0.5 x 106 cells/ml; split culture 1:2 every 2-3 days; viability may vary between 70% and 90%
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 35 hours
Harvest:
saturation density at about 0.5-1.0 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO

DSMZ Scientific Data:
Mycoplasma:
contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
CD2 -, CD3 -, CD13 -, CD14 -, CD15 +, CD19 -, CD25 -, CD30 +, CD34 -;


Fingerprint:
multiplex PCR of minisatellite markers revealed a unique DNA profile
Species:
originally confirmed as human with IEF of AST, MDH
Cytogenetics:
human flat-moded hypertetraploid karyotype with 10% polyploidy - 94(75-99)<4n>XXXX, +2, -5, +6, -9, -9, +12, -13, +17, +4mar, +2r/dmin, del(1)(q13), der(2)t(2;4)(p14;q21)x2-3, add(5)(p11), add(6)(q24/25)x2, add(7)(q35)x2, del(7)(p14), add(9)(p22), del(11)(q22.3), del(12)(q21)x1-2, add(13)(p11)x2, add(14)(q32)x2, der(16)t(16;?)(q24;?)t(1;?)(q11;?), del(17)(p12), del(21)(q21.2)x2 - resembles published karyotype
Molec. Genetics:
expression of fusion gene ELMO1-SLCO3A1 confirmed by RT-PCR
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -


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