U-2197 cell line细胞株细胞系 BioVector NTCC质粒载体菌种细胞基因保藏中心

U-2197 cell line细胞株细胞系 BioVector NTCC质粒载体菌种细胞基因保藏中心
Cell line:
U-2197
DSMZ no.:
ACC 406
Species:
human (Homo sapiens)
Cell type:
malignant fibrous histiocytoma
Origin:
established from the subcutaneous malignant fibrous histiocytoma of a 79-year-old Caucasian man in 1984 (third recurrence after repeated surgery, radially invading the thumb extensor); described in the literature to express high levels of PDGF-A which is not secreted
Biosafety level:
1

DSMZ Cell Culture Data:
Morphology:
adherent fibroblastoid large polygonal cells with extensive lamellae;

Medium:
80% MEM (with Earle's salts) + 20% h.i. FBS + 0.135% Na-bicarbonate
Subculture:
seed out at ca. 1 x 106 cells/80 cm2 in 10 ml medium; split confluent culture 1:2 to 1:3 once a week using trypsin/EDTA; maintain at 6 x 106 cells/175 cm2
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 100-120 hours
Harvest:
cell harvest of ca. 8 x 106 cells/175 cm2; maximal density of 10 x 106 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO

DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
cytokeratin -, desmin -, endothel -, GFAP (+), neurofilament -, vimentin +
Fingerprint:
multiplex PCR of minisatellite markers revealed a unique DNA profile
Species:
confirmed as human with IEF of AST, NP
Cytogenetics:
human hypertetraploid karyotype with hypotriploid sideline and 20% polyploidy - 100(92-101)<4n>XXXX, -2, -4, +6, +6, +7, -8, -9, -9, +14, +14, -15, -15, -16, -16, +19, -20, -20, -21, -21, +13mar, i(Xq)x2, add(X)(p11)x2, del(1)(q11)x2, del(4)(p11), add(6)(q15)x2, i(6p)x2-4, add(7)(q35)x2, add(7)(p14)x2, del(7)(p11), add(12)(q24)x2, add(13)(p11)x2, add(13)(p11)x2, add(19)(q13), del(19)(q13)x2 - resembles published karyotype
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -


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