pDHB1-Nef plasmid vector质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心
- 价 格：¥49865
- 货 号：pDHB1-Nef plasmid vector质粒载体
- 产 地：北京
- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作微信:1843439339 (QQ同号)
邮件：Biovector@163.com
手机：18901268599
地址：北京
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pDHB1-Nef plasmid vector质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心
The respective pPR3 prey vector DNAs of each of the hits was isolated and retransformed into yeast strain NMY51 either with the bait plasmid (pDHB1-Nef) or with a control bait (pDHB1-largeT) encoding an Ost4p-largeT-Cub-LexA-VP16- fusion. Retransformants were analyzed for their growth and phenotype on plates lacking leucine and tryptophane (SD -leu -trp) or on plates additionally lacking histidine and adenine (SD -leu -trp -his -ade) as well as for their β-galactosidase activity as described in more detail in the DUALhunter kit handbook (DUALsystems). Only those who showed a prominent β-galactosidase activity when coexpressed with the Nef-bait, but not when coexpressed with the largeT control bait, were considered to be Nef dependent positive ligands.
【Supplier来源】BioVector NTCC Inc.
TEL：+86-010-53513060
【Website网址】 http://www.biovector.net
The respective pPR3 prey vector DNAs of each of the hits was isolated and retransformed into yeast strain NMY51 either with the bait plasmid (pDHB1-Nef) or with a control bait (pDHB1-largeT) encoding an Ost4p-largeT-Cub-LexA-VP16- fusion. Retransformants were analyzed for their growth and phenotype on plates lacking leucine and tryptophane (SD -leu -trp) or on plates additionally lacking histidine and adenine (SD -leu -trp -his -ade) as well as for their β-galactosidase activity as described in more detail in the DUALhunter kit handbook (DUALsystems). Only those who showed a prominent β-galactosidase activity when coexpressed with the Nef-bait, but not when coexpressed with the largeT control bait, were considered to be Nef dependent positive ligands.
【Supplier来源】BioVector NTCC Inc.
TEL：+86-010-53513060
【Website网址】 http://www.biovector.net
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