Agrobacterium tumefaciens KYC55 BioVector NTCC质粒载体菌种细胞基因保藏中心

Agrobacterium tumefaciens KYC55 BioVector NTCC质粒载体菌种细胞基因保藏中心
A. tumefaciens strain KYC55, a derivative of strain R10 that lacks the Ti plasmid and therefore cannot produce detectable autoinducers. It is used for detection of N-Acylhomoserine Lactone-type quorum-sensing (QS) molecules.
A sensitive and rapid double-layer plate method using biosensor strain Agrobacterium tumefaciens KYC55 was developed to detect the N-acylhomoserine lactone (AHL)-related compounds in bacteria.
Two AHLs bacterial biosensors, Chromobacterium violaceum CV026 and Agrobacterium tumefaciens KYC55 which respond to short-chain and long-chain AHLs, respectively, were used in the preliminary screening of AHL produced by the bacteria isolated from spoiled instant sea cucumber. Agar plate diffusion assay was used in the AHL screening process that multiple AHLs-producting bacteria were screened in one culture dish by Anbazhagan et al. , to avoid the false positives due to possible contamination between these isolated strains in one dish, some modifications were carried out. Briefly,
the bacterial strain to be tested for the production of AHLs as well as C. violaceum CV026 were streaked
parallel to each other on a LB agar plate. Detection of AHLs production was also carried using
A. tumefaciens KYC55, and the assay was basically performed in the same way, except that the agar
was supplemented with 50 µg/mL X-gal. In both cases, the plates were incubated at 28 ◦C for overnight. Production of exogenous AHLs was indicated by the formation of the purple pigment violacein or blue coloration by β-galactosidase activity

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